辽宁汉族人群HLA-B等位基因多态性的分布

目的调查辽宁汉族人群HLA-B等位基因的遗传多态性。方法用聚合酶链反应．序列特异性引物方法对辽宁8962名健康无关汉族人进行HLA-B等位基因分型，计算HLA-B等位基因频率并与不同人群HLA-B等位基因的多态性进行比较。结果共检出HLA-B等位基因34种，其中B＊15（14．42％）、B＊40（14．33％）和B＊13（11．99％）基因频率分布较高，B＊82、B＊83等位基因未检出；HLA-B座位特异性49种。该人群与南北方汉族人群、日本人、黑人和白人分别进行X^2检验差异有统计学意义，X^2值分别为1584．799、72．145、1393．339、7406．288和5311．947。结论辽宁汉族人群HLA-B基因多态性分布有其自身特点，它的遗传特征不同于既往的南、北方汉族。

Distribution of HLA-B allele polymorphim of 8962 Han population in Liaoning of China

Objective To investigate the distribution of genetic polymorphism of HLA-B locus in Liaoning Han population. Methods The technique of polymerase chain reaction-sequence specific primers (PCR-SSP) was adopted in genotyping a sample of 8962 unrelated healthy individuals collected from a Chinese Han population in Liaoning area. The allele frequency of HLA-B was calculated and was compared with the results from other population. Results Thirty-four HLA-B alleles were detected. The more common HLA-B allele included B*15(14.42%),B*40(14.33%) and B*13(11.99%). The B*82 and B*83 were absent. Forty-nine HLA-B antigens were detected. Conclusion The HLA-B allele polymorphism distribution of Liaoning Han population exhibits its own characteristic and the difference from old south and north Han population in China.