微量元素矿化液促进釉质龋再矿化层形成机理的研究

目的：观察不同成份的再矿化液对人工釉质龋表层下脱矿的再矿化作用和再矿化层的形成机理。方法：选择牛牙３６颗于乳酸凝胶中形成人工釉质龋、两周后将以上脱矿釉质块随机分成４组样本，其中３组为实验组，１例为对照组。实验组釉质块分别置无氟、含氟及含有氟、钙、磷、锡等微量元素的再矿化液中；对照组用去离子水代替再矿化液。借助Ｘ光电子能谱、扫描电镜、偏光显微镜等，观察不同的矿化液处理人工釉质龋后脱矿釉质表面沉积物的

A Study of the Mechanism of Effects of Solution Containing Trace Elements on Remineralization Layer Formation of Enamel Carious Lesions

Objective: To study the effects of several remineralization solutions with different composition on the artificial demineralization lesions of bovine enamel and the mechanism of remineralization layer formation. Methods: 36 bovine teeth were selected and artificial carious lesions were formed with lactic acid gel. Then all specimens were divided into 4 groups which contained no fluoride, fluoride, fluoride calciulm phospate tin, and de ionic water separately, and the de ionic group was taken as control. The structure and composition of the sediments on the enamel surface were evaluated and detected by X ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Histological change of enamel section was examined by polarized microscopy. Results: Compared with the control group, all the solutions had remineralization effects, especially the solutions containing trace elements. XPS showed that there were sediments formed on the surface of carious lesion, the compositions of the sediments were CaF-2, HAP and non specifically absorbed fluoride (NSAF). Irregular, crystal deposits were also found under SEM on the enamel surface. The polarized microscopy results showed that the observed demineralization areas decreased after exposure to the solu tions. Conclusion: Solutions containing fluoride and trace elements were effective on remineralization. The possible mechanism is that CaF-2 and NSAF, instead of FA formed in the remineralization layer. Moreover, the remineralization effects of the fluoride solutions containing trace elements are more outstanding than that without trace elements. The potential mechanism is the synergistic action of fluoride and trace elements.;