核糖核酸预防日本血吸虫病病兔肝纤维化的效果

目的：观察核糖核酸（ＲＮＡ）对日本血吸虫病病兔肝纤维化的阻抑效果。方法：５４只３月龄家兔随机均分为正常组、感染组及ＲＮＡ组。于肌注ＲＮＡ后第４０ｄ、７０ｄ及１００ｄ，各组随机选６只兔抽血及取肝组织，通过光镜、电镜观察其组织病理变化，放射免疫法测定血浆ＨＡ含量，紫外分光光度法测定肝组织ＨＹＰ，ＩＢＡＳ图像分析仪测定肝组织胶原纤维分布面积百分比，用潘孺孙法测定肝组织胶原纤维含量。结果：与感染组相比较，ＲＮＡ组胶原纤维未见明显增加，而肝细胞核仁增大，粗面内质网及其附着颗粒增多，贮脂细胞周围胶原纤维减少，血浆ＨＡ、肝组织ＨＹＰ、胶原纤维分布面积百分比及胶原纤维含量均显著降低。结论：ＲＮＡ对日本血吸虫病病兔肝纤维化具有早期预防作用。

EFFECT OF RNA AGAINST HEPATIC FIBROSIS IN RABBITS INFECTED WITH SCHISTOSOMAJAPONICUM

AIM: To study the effect of ribonucleic acid (RNA) against schistosomal hepatic fibosis in rabbits. METHODS: 54 rabbits were randomly and equally divided into normal group, infected group, and RNA group. On the 40,70,100th day after RNA i.m. injection, 6 rabbits from each of the above 3 groups were killed and their plasma and liver were examined by way of biochemistry and pathology. RESULTS: Compared with the infected group the collagen fiber of liver in RNA group was less produced, the hepatic cellular nucleolus was enlarged, the number of rough endoplasmic reticulum (RER) and the granules on the RER were increased, the collagen fiber around fat storing cell was decreased, the content of hyaluronic acid in plasma, the hydroxyproline level in liver tissue, the percentage of collagen fiber distribution and the collagen fiber level in liver tissue of RNA group were significantly reduced. CONCLUSION: RNA might play a role in the prevention of schistosomal hepatic fibrosis.