异盘并殖吸虫在大鼠体内发育的实验研究

目的：探讨异盘并殖吸虫在大鼠体内发育的多态性及其主要寄生部位，确定大鼠的宿主性质。方法：囊蚴经口感染大鼠后，用生理盐水逸出法，分离组织中童虫或直接从肺和肝脏虫囊内取虫，测量虫体大小，染色并摄影。结果：异盘并殖吸虫感染大鼠１４ｄ时，虫体侵入肝脏、肌肉和腹腔；３０ｄ时，发育出现分化。体腔中虫体发育迅速，成为具有生殖器官雏形的大型童虫，肌肉中虫体发育迟缓；感染７０ｄ以后，大鼠的肺、肝和胸壁有虫囊形成，囊内虫体发育成熟且产卵。９只大鼠感染７０ｄ后，其中７只在肝脏检获多个虫囊，最多达１１个虫囊。囊内虫体发育良好，成熟率为７８．６％－１００％，最大虫体为１６ｍｍ×８ｍｍ。肝脏严重受损，表面凹凸不平，纤维结缔组织增生，肝细胞变性坏死。分布于肌肉中的虫体占３２．６％－８２．４％，始终保持小型童虫状态。感染后１５６ｄ，虫体仍无发育，其大小为９１４．８７μｍ±７８．２１μｍ×５２５．３４μｍ±７６．２６μｍ，排泄囊内充满黑色颗粒。结论：异盘并殖吸虫在大鼠体内发育呈多态性且多部位寄生，大鼠为其终宿主及其转续宿主。

EXPERIMENTAL STUDIES ON DEVELOPMENT OF PARAGONIMUS HETEROTREMUS IN RATS

AIM: To study the polymorphism of the development of Paragonimus heterotremus in rats and to ascertain the host nature of the rat. METHODS: SD and Wistar rats were each infected orally with 100 metacercariae of P.heterotremus . The distribution and development of the worms recovered from rats 14-156 days after infection were observed. RESULTS:Thirty days after infection, reproductive organs appeared in the worms recovered from the body cavities.Seventy days after infection,worm cysts were found in the lungs,livers and pleural wall, the worms in the cysts matured but the worms detected in the muscles were still stunted on the 156 th day after infection. CONCLUSION:Rat is the definitive host and paratenic host of P.heterotremus . The development of P.heterotremus is polymorphic.