| 日本血吸虫童虫培养细胞SOD和MDA动态变化的研究 |
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| 引用本文： | 蒋明森,陈晓蓓,董惠芬,杨明义,倪永晖,周述龙.日本血吸虫童虫培养细胞SOD和MDA动态变化的研究[J].中国寄生虫学与寄生虫病杂志,1998,16(5):357-359. |
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| 作者姓名： | 蒋明森 陈晓蓓 董惠芬 杨明义 倪永晖 周述龙 |
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| 作者单位： | 湖北医科大学寄生虫学教研室，湖北医科大学附属第一医院传染科 |
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| 基金项目： | 湖北省教委和卫生厅资助 |
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| 摘 要： | 目的：观察日本血吸虫童虫细胞在体外培养过程中超氧化物歧化酶（SOD）和丙二醛（MDA）含量的动态变化，探讨培养细胞的退化过程。方法：应用邻苯三酚自氧化法及硫代巴比妥酸法分别测定童虫细胞在培养0－54d过程中SOD和MDA含量的变化。结果：在培养第12dSOD出现一个高峰，以后一直处于低平状态。MDA在培养第18d和第48d分别各有一个高峰，其后均逐渐降低。结论：日本血吸虫童虫培养细胞呈逐步退化过程。
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| 关 键 词： | 日本血吸虫 童虫 培养细胞 SOD MDA |
STUDIES ON DYNAMIC CHANGES OF SOD AND MDA IN CULTURED CELLS FROM SCHISTOSOMA JAPONICUM SCHISTOSOMULA |
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| Jiang Mingsen,Chen Xiaobei,Dong Huifen,Yang Mingyi,Ni Yonghui,Zhou Shulong.STUDIES ON DYNAMIC CHANGES OF SOD AND MDA IN CULTURED CELLS FROM SCHISTOSOMA JAPONICUM SCHISTOSOMULA[J].Chinese Journal of Parasitology and Parasitic Diseases,1998,16(5):357-359. |
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| Authors: | Jiang Mingsen Chen Xiaobei Dong Huifen Yang Mingyi Ni Yonghui Zhou Shulong |
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| Institution: | Department of Medical Parasitology, Research Laboratory of Schistosomiasis, Hubei Medical University, Wuhan 430071. |
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| Abstract: | AIM:To observe the dynamic changes of superoxide dismutase(SOD) and maldondialdehyde(MDA) in cultured cells from Schistosoma japonicum schistosomula and to explore the degeneration process of cultured cells. METHODS: Pyrogallol method and thiobarbituric acid method were used to determinate the levels of SOD and MDA , respectively,in the cells cultured for 0-54 days. RESULTS: SOD in the cells reached a peak level on d12 of the early stage of culture and then decreased . The MDA levels in the cultures cells ascended gradually, and had two peaks on d18 and d48, during the period of culture. CONCLUSION:The cultured cells from S. japonicum schistosomula degenerated progressively. |
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| Keywords: | Schistosoma japonicum schistosomula cultured cells SOD MDA |
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