| 实时定量PCR阵列技术在规模化检测基因表达中的应用 |
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| 引用本文： | 张艳春,任长虹,高艳,屈武斌,张成岗. 实时定量PCR阵列技术在规模化检测基因表达中的应用[J]. 军事医学科学院院刊, 2010, 34(6): 589-591 |
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| 作者姓名： | 张艳春 任长虹 高艳 屈武斌 张成岗 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所蛋白质组学国家重点实验室,北京100850 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家科技重大专项课题，国家自然科学基金课题 |
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| 摘 要： | 实时定量PCR阵列技术（RQ-PCR-array）以实时定量PCR（RQ-PCR）技术为核心并结合阵列（array）技术,能够比较可靠、准确地检测信号转导、疾病相关通路等过程相关的基因表达图谱,在规模化检测基因表达方面具有高通量的显著优势,已广泛应用于基础研究和临床。如能够结合多重PCR技术,有可能进一步达到超高通量基因表达检测水平。本文对该技术在规模化检测基因表达中的研究进展进行综述。
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| 关 键 词： | 实时定量PCR阵列 基因表达 高通量 超高通量 信号转导 |
Application of real-time quantitative PCR array technique in large scale analysis of gene expression |
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| ZHANG Yan-chun,REN Chang-hong,GAO Yan,QU Wu-bin,ZHANG Cheng-gang. Application of real-time quantitative PCR array technique in large scale analysis of gene expression[J]. Bulletin of the Academy of Military Medical Sciences, 2010, 34(6): 589-591 |
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| Authors: | ZHANG Yan-chun REN Chang-hong GAO Yan QU Wu-bin ZHANG Cheng-gang |
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| Affiliation: | (Beijing Institute of Radiation Medicine,State Key Laboratory of Proteomics,Beijing 100850,China) |
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| Abstract: | The real-time quantitative PCR array technique（RQ-PCR-array）,based on the RQ-PCR and combined with the array approach,can be used to analyze the expression of a large number of genes concerning signal transduction,signal pathway with diseases.The most fascinating is the large-scale genes expression analysis ability by using this novel technique,which is especially important for basic and clinical studies.In addition,if combined with multiplex PCR,the RQ-PCR array will probably contribute to super high-throughput level analysis of the gene expression.In this paper,the research progress in this field is reviewed. |
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| Keywords: | real-time quantitative PCR array gene expression high-throughput super high-throughput signal transduction |
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