广西虎纹捕鸟蜘蛛毒素对胶质瘤细胞株U251增殖抑制作用的研究

目的 探讨广西虎纹捕鸟蜘蛛毒素对胶质瘤细胞株U251增殖抑制作用,并初步阐明其作用机制. 方法 以含10%胎牛血清RPMIl640培养液湿化孵育培养U251细胞.采用MTT法通过抑制率评价蜘蛛毒素对胶质瘤细胞的细胞毒作用,应用倒置相差显微镜及HE染色观察细胞形态及细胞核变化,采用TUNEL法检测蜘蛛毒素对U251细胞凋亡的影响. 结果 蜘蛛毒素能够抑制胶质瘤细胞株U251的增殖.其48 h及72 h的半量抑制浓度为82.60μg/mL和71.12μg/mL,且呈明显的量效及时效关系.HE染色观察到胶质瘤细胞核同缩、碎裂的凋亡征象.TUNEL法染色可见凋亡的胶质瘤细胞核染成棕黄色. 结论 广西虎纹捕鸟蜘蛛毒素能够抑制胶质瘤细胞U251增殖,其抑制作用可能是通过诱导胶质瘤细胞凋亡实现.

Inhibition by Guangxi Selenocosmia huwena toxins on human glioma cell strain U251

Objective To investigate the inhibition by Guangxi Selenocosmia huwena toxins on human glioma cell strain U251 and to interpret its possible mechanisms, with regard to initial clarify its mechanism. Methods Culture medium of Containing 10% fetal bovine serum wet RPMI1640 of U251 cells incubated with culture, MTT was involved indetecting the cytotoxic effects. HE staining and inverted phase contrast microscope was used to detected the change of cell shape and cellular nucleus shape. TUNEL was used to detected apoptosis ofhuman glioma cell strain U251. Results The proliferation of U251 was obviously inhibited by Guangxi Selenocosmia huwena toxins in dose- and time- dependent manner. The inhibitive effect of Guangxi Selenocosmia huwena toxins on U251 was obvious, with the Two half-inhibitory concentration of 82.60 μg/mL and 71.12 μg/mL at 48 h and 72 h respectively, This depressanteffct offer conspicuous dose-effect relationship. We could observesome cell nucleus became to pycnosis and nuclear fragmentation (cell apoptosis). We also could dye apoptosis cell nucleus in brown. Conclusion Guangxi Selenocosmia huwena toxins can inhibit theproliferation of glioma U251 which was implemented through the inducement ofglioma apoptosis.