| 青蒿琥酯对不同急性白血病细胞株凋亡抑制的实验研究 |
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| 引用本文： | 陈均法,沈一平,郑智茵,陈小红,沈建平,刘永林,虞荣喜,周郁鸿.青蒿琥酯对不同急性白血病细胞株凋亡抑制的实验研究[J].浙江中医药大学学报,2011,35(1):69-71. |
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| 作者姓名： | 陈均法 沈一平 郑智茵 陈小红 沈建平 刘永林 虞荣喜 周郁鸿 |
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| 作者单位： | 浙江中医药大学附属第一医院,杭州,310006 |
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| 基金项目： | 浙江省中医药科技计划资助项目 |
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| 摘 要： | 目的]观察青蒿琥酯对不同急性白血病细胞株的增殖抑制作用及凋亡机制探讨。方法]应用不同浓度青蒿琥酯作用U937、HL60、Jurkat细胞株,MTT法检测抑制率;细胞形态学、DNA琼脂糖凝胶电泳、流式细胞术检测细胞凋亡;Western-blot法检测细胞凋亡过程中Bcl-2、Bax、Caspase 8和ICAD蛋白表达的变化。结果]青蒿琥酯能够明显抑制U937、HL60、Jurkat细胞增殖;8μg/ml以上浓度青蒿琥酯作用三种细胞24h、48h、72h抑制率均为Jurkat〉HL60〉U937(均P<0.01);其增殖抑制作用与诱导细胞凋亡有关;随药物浓度的增高Bcl-2和ICAD蛋白表达量下降,而Bax蛋白表达上调,Caspase8蛋白出现明显的剪切激活带。结论]青蒿琥酯既能通过线粒体途径又能通过外源性途径诱导急性白血病细胞凋亡,对Jurkat的抑制最强。
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| 关 键 词： | 青蒿琥酯 白血病 细胞凋亡 |
Apoptosis and Inhibitory Effects of Artesunate on Different Cell Strains of Acute Leukemia |
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| Chen Junfa,Shen Yiping,Zheng Zhiyin,et al The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou.Apoptosis and Inhibitory Effects of Artesunate on Different Cell Strains of Acute Leukemia[J].Journal of Zhejiang University of Traditional Chinese Medicine,2011,35(1):69-71. |
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| Authors: | Chen Junfa Shen Yiping Zheng Zhiyin The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine Hangzhou |
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| Institution: | Chen Junfa,Shen Yiping,Zheng Zhiyin,et al The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou(310006) |
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| Abstract: | Objective]To investigate the proliferation and inhibitory action of artesunate on different cell strains of acute leukemia as well as its apoptosis mechanism.Methods]Cell strains of U937,HL60 and Jurkat were treated with different doses of artesunate.Inhibition ratio was detected with MTT method,and apoptosis was detected with cell morphology,DNA agarose gel electrophoresis,and flow cytometry.The changes of the protein expression of Bcl-2,Bax,Caspase 8 and ICAD were detected with Western-blot during apopt... |
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| Keywords: | artesunate leukemia apoptosis |
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