| 雷帕霉素对胰腺癌细胞株SW1990生长和凋亡的影响 |
| |
| 引用本文： | 殷红,朱毅,王宇岭,高远. 雷帕霉素对胰腺癌细胞株SW1990生长和凋亡的影响[J]. 肿瘤, 2011, 31(1): 49-52. DOI: 10.3781/j.issn.1000-7431.2011.01.010 |
| |
| 作者姓名： | 殷红 朱毅 王宇岭 高远 |
| |
| 作者单位： | 1. 苏州大学附属第一医院肿瘤科,苏州,215006
2. 南京医科大学第一附属医院普外科,南京,210029 |
| |
| 摘 要： | 目的:探讨雷帕霉素(rapamycin,RAPA)在体外对胰腺癌细胞株SW1990生长和凋亡的影响及其可能的机制。方法:用5、10、20、30、40和50nmol/L RAPA作用SW1990细胞24、48和72h,MTT法检测细胞的生长抑制率,FCM法检测细胞凋亡和细胞周期,Western印迹法检测SW1990细胞中bcl-2、bcl-xL、bax和survivin蛋白的表达。结果:MTT和FCM检测结果显示,随着RAPA浓度的增加和作用时间的延长,SW1990细胞的生长抑制率和细胞凋亡率逐渐增加,与对照组比较,差异有统计学意义(P<0.05);各浓度RAPA作用细胞24h后,随着浓度的增加,G0/G1期细胞逐渐增多,S期和G2/M期细胞逐渐减少,细胞阻滞于G1期,与对照组比较,差异有统计学意义(P<0.05);Western印迹法检测结果显示,50nmol/L RAPA作用SW1990细胞后,bax表达明显增加,bcl-2、bcl-xL、survivin、bcl-xL/bax和bcl-2/bax表达明显降低,差异有统计学意义(P<0.05)。结论:RAPA可显著抑制SW1990细胞增殖和诱导细胞凋亡,其机制可能与凋亡调控基因bcl-2、bcl-xL、bax和survivin表达水平变化有关。
|
| 关 键 词： | 胰腺肿瘤 雷帕霉素 细胞周期 细胞凋亡 凋亡调节蛋白质类 细胞,SW1990 |
Effects of rapamycin on cell growth and apoptosis of pancreatic carcinoma SW1990 cells |
| |
| YIN Hong,ZHU Yi,WANG Yu-ling,GAO Yuan. Effects of rapamycin on cell growth and apoptosis of pancreatic carcinoma SW1990 cells[J]. Tumor, 2011, 31(1): 49-52. DOI: 10.3781/j.issn.1000-7431.2011.01.010 |
| |
| Authors: | YIN Hong ZHU Yi WANG Yu-ling GAO Yuan |
| |
| Affiliation: | YIN Hong1,ZHU Yi2,WANG Yu-ling1,GAO Yuan1 1.Department of Oncology,First Hospital Affiliated to Soochow University,Suzhou 215006,China,2.Department of General Surgery,First Affiliated Hospital,Nanjing Medical University,Nanjing 210029 |
| |
| Abstract: | Objective:To investigate the effects of rapamycin on cell growth and apoptosis of pancreatic carcinoma SW1990 cells in vitro,and to elucidate its possible mechanism.Methods:MTT assay was used to detect the growth inhibitory rate of SW1990 cells treated with 5,10,20,30,40 and 50 nmol/L rapamycin for 24,48 and 72 h,respectively.Cell apoptosis and cell cycle were detected by flow cytometry(FCM).The expressions of bcl-2,bcl-xL,bax and survivin were measured by Western blotting.Results:The inhibitory rate and ap... |
| |
| Keywords: | Pancreatic neoplasms Rapamycin Cell cycle Apoptosis Apoptosis regulatory proteins Cell SW1990 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
