| 三叶青提取物对白血病HL60细胞增殖抑制作用研究 |
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| 引用本文： | 徐彩菊,吴平国,姚亚萍,孟佳,鹿伟,陈玉满,白宁宁,丁钢强.三叶青提取物对白血病HL60细胞增殖抑制作用研究[J].浙江预防医学,2011,23(4):20-22. |
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| 作者姓名： | 徐彩菊 吴平国 姚亚萍 孟佳 鹿伟 陈玉满 白宁宁 丁钢强 |
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| 作者单位： | 1. 浙江省疾病预防控制中心毒理所,浙江杭州,310051
2. 湖南科技大学 |
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| 基金项目： | 浙江省中医药科研基金项目 |
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| 摘 要： | 目的探讨三叶青提取物对白血病HL60细胞的增殖、凋亡的影响。方法将不同浓度的受试物作用于体外培养的HL60细胞,用四氮唑蓝比色法（MTT）检测细胞生长抑制率,应用流式细胞仪观察细胞凋亡。结果受试物处理24 h后6.25、12.50、25.00、50.00、100.00μg/m l组,受试物处理48、72 h后所有浓度组HL60细胞的增殖率和凋亡率均呈不同程度的下降或上升,差异均有统计学意义。结论三叶青提取物能有效抑制体外HL60细胞的增殖,诱导HL60细胞凋亡。
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| 关 键 词： | 三叶青提取物 白血病细胞HL60 细胞增殖 细胞凋亡 |
A Study on Inhibitory Effect of Extracts from Tetrastigma Hemsleyanum Diels et.Gilg on Proliferation of HL60 Cell Line |
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| Institution: | Xu Cai-ju,Wu Ping-guo,Yao Ya-ping,et al.The Center for Disease Control and Prevention of Zhejiang Province,Hangzhou,Zhejiang,310051,China. |
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| Abstract: | Objective To investigate the inhibitory effect of extracts from Tetrastigma Hemsleyanum Diels et.Gilg（TDG） on human leukemia HL60 cells.Methods Various concentrations of extracts from TDG were given to HL60 cells in vitro.The inhibitory ratio of the cells was measured by MTT assay.The apoptosis was observed by flow cytometry（FCM）.Results All concentrations of the extracts from TDG could inhibit the growth of HL60 cells and induce apoptosis.Conclusion The extracts from TDG can significantly inhibit proliferation of HL60 cells and induce apoptosis. |
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| Keywords: | Extracts of the tetrastigma hemsleyanum diels et gilg Leukemia HL60 cell line Cellular proliferation Apoptosis |
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