| 小干扰RNA特异性沉默泛素特异肽酶22基因对膀胱癌细胞增殖的抑制作用 |
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| 引用本文： | 吕磊,曾甫清,唐冬,王智宇,蒋国松,肖行远.小干扰RNA特异性沉默泛素特异肽酶22基因对膀胱癌细胞增殖的抑制作用[J].肿瘤,2011,31(1):17-21. |
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| 作者姓名： | 吕磊 曾甫清 唐冬 王智宇 蒋国松 肖行远 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院泌尿外科,武汉,430022 |
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| 摘 要： | 目的:研究小RNA干扰泛素特异肽酶22(ubiquitin specific peptidase22,USP22)基因表达对膀胱癌EJ细胞增殖的影响。方法:设计并合成针对USP22基因的3条特异性小干扰RNA(small interfering RNA,siRNA)及阴性siRNA,并同时设立空白对照,然后用脂质体Translipid转染EJ细胞。通过实时荧光定量RT-PCR和Western印迹法分别检测转染前后EJ细胞中USP22mRNA和蛋白表达水平,MTT法和克隆形成实验检测细胞增殖率,FCM法检测细胞周期分布。结果:3条针对USP22基因的特异性siRNA均能抑制USP22mRNA和蛋白的表达,其中USP22-siRNA-1的沉默效率最高。转染USP22-siRNA-148h后,EJ细胞中USP22mRNA和蛋白表达水平分别下调65%和80%,而且细胞增殖明显受到抑制,细胞周期被阻滞于G1期。结论:USP22特异性siRNA能够有效沉默USP22基因表达,并显著抑制膀胱癌EJ细胞增殖。推测USP22基因可能成为治疗膀胱癌的一个新靶点。
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| 关 键 词： | 膀胱肿瘤 泛素巯基酯酶 RNA 小分子干扰 细胞增殖 细胞周期 泛素特异肽酶22 |
The inhibitory effect of specific small interfering RNA against ubiquitin specific peptidase 22 gene on cell proliferation of bladder cancer cells |
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| L Lei,ZENG Fu-qing,TANG Dong,WANG Zhi-yu,JIANG Guo-song,XIAO Xing-yuan.The inhibitory effect of specific small interfering RNA against ubiquitin specific peptidase 22 gene on cell proliferation of bladder cancer cells[J].Tumor,2011,31(1):17-21. |
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| Authors: | L Lei ZENG Fu-qing TANG Dong WANG Zhi-yu JIANG Guo-song XIAO Xing-yuan |
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| Institution: | L(U) Lei,ZENG Fu-qing,TANG Dong,WANG Zhi-yu,JIANG Guo-song,XIAO Xing-yuan |
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| Abstract: | Objective: To investigate the effect of specific small interfering RNA (siRNA) against ubiquitin specific peptidase 22(USP22) gene on the proliferation of bladder cancer cell line EJ.Methods: Three specific USP22 siRNAs and one negative siRNA were individually transfected into EJ cells for 48 h .The expression levels of USP22 mRNA and protein were analyzed by real-time fluorescence quantitative PCR (qRT-PCR) and Western blotting,respectively.The cell proliferation was determined by MTT assay and plate clone... |
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| Keywords: | Bladder neoplasms Ubiquitin thiolesterase RNA small interfering Cell proliferation Cell cycle Ubiquitin specific peptidase 22 |
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