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| 人参皂苷Rg3对小鼠B16黑素瘤细胞侵袭、转移及MMP-9表达的影响 | |
| 引用本文： | 姜新,辛颖,许天敏,曲雅勤. 人参皂苷Rg3对小鼠B16黑素瘤细胞侵袭、转移及MMP-9表达的影响[J]. 肿瘤, 2011, 31(2): 117-121. DOI: 10.3781/j.issn.1000-7431.2011.02.005 |
| 作者姓名： | 姜新 辛颖 许天敏 曲雅勤 |
| 作者单位： | 1. 吉林大学第一医院肿瘤中心放疗科,长春,130021 2. 吉林大学病理生物学教育部重点实验室,长春,130021 3. 吉林大学第二医院妇产科,长春,130021 |
| 基金项目： | 吉林省科技发展计划资助项目 |
| 摘 要： | 目的:观察人参皂苷Rg3(简称Rg3)对小鼠黑素瘤细胞B16的肺转移、侵袭能力及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达的影响,探讨其抗肿瘤转移的作用机制。方法:体内建立小鼠自发肺转移和实体瘤模型,观察腹腔注射不同剂量的Rg3(对照组给予0.9%氯化钠溶液)后,肺部肿瘤转移灶的数目,并检测实体瘤中MMP-9蛋白的表达情况。采用Boyden小室侵袭实验及免疫细胞化学染色法检测Rg3对肿瘤细胞体处侵袭能力及MMP-9表达的影响。结果:采用不同剂量的Rg3(0.3、1.0和3.0mg/kg)治疗后,小鼠肺部转移灶的数目均较少,肿瘤组织中MMP-9的表达水平降低,与对照组比较差异有统计学意义(P<0.05)。在体外,2.5和5.0μg/mLRg3治疗组中侵袭穿过人工基膜的B16细胞数目明显少于对照组(P<0.01),且5.0μg/mLRg3可抑制肿瘤细胞中MMP-9的表达。结论:Rg3能够抑制小鼠黑素瘤细胞的肺转移,其抗肿瘤转移作用可能是通过降低肿瘤细胞中MMP-9的表达水平及细胞侵袭能力来实现的。 |
| 关 键 词： | 黑素瘤,实验性 人参皂苷类 肿瘤转移 肿瘤浸润 基质金属蛋白酶 |
Effects of ginsenoside Rg3 on the invasion and metastasis of mouse melanoma cell line B 16 as well as the expression of MMP-9 |
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| JIANG Xin,XIN Ying,XU Tian-min,QU Ya-qin. Effects of ginsenoside Rg3 on the invasion and metastasis of mouse melanoma cell line B 16 as well as the expression of MMP-9[J]. Tumor, 2011, 31(2): 117-121. DOI: 10.3781/j.issn.1000-7431.2011.02.005 | |
| Authors: | JIANG Xin XIN Ying XU Tian-min QU Ya-qin |
| Affiliation: | JIANG Xin1,XIN Ying2,XU Tian-min3,QU Ya-qin1 1.Department of Radiotherapy,Cancer Center,First Hospital,2.Ministry of Education Key Laboratory of Pathobiology,3.Department of Obstetrics and Gynecology,Second Hospital,Jilin University,Changchun 130021,China |
| Abstract: | Objective:To observe the effects of ginsenoside Rg3 on the invasive and metastatic potentials of mouse melanomas B16 cells in vitro and in vivo,as well as the expression of matrix metalloproteinase-9(MMP-9),and to investigate the underlying mechanisms.Methods:The grafted melanoma and its spontaneous lung metastasis model in mice was established by transplanting with B16 cells.The number of metastatic lesions in the lung was calculated,and the expression of MMP-9 in melanoma tissues was detected by immunohis... |
| Keywords: | Melanoma experimental Ginsenosides Neoplasm metastasis Neoplasm invasiveness Matrix metalloproteinase |
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