3aicalin inhibits colistin sulfate-induced apoptosis of PC12 cells |
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Authors: | Hong Jiang Pengfei Lv Jichang Li Hongjun Wang Tiezhong Zhou Yingzi Liu Wei Lin |
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Affiliation: | [1]Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China [2]Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China [3]Scientific Research Department, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China |
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Abstract: | Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress co-listin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5–500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin (25, 50 and 100 μg/mL), and exposed to 125 μg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhi-bited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity. |
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Keywords: | neural regeneration traditional Chinese medicine baicalin colistin sulfate PC12 cells apoptosis caspase-3 lactate dehydrogenase grants-supported paper neuroregeneration |
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