巨噬细胞在泡沫细胞化过程中的Ca2+变化

我室曾用Ｃ＿（５７）ＢＬ／６Ｊ品系小鼠腹膜巨噬细胞与１０ｍｇ·Ｌ￣（－１）氧化低密度脂蛋白孵育４天，建立了出现在早期动脉粥样硬化损伤中的巨噬细胞源性泡沫细胞的病理细胞模型，本文报道应用Ｃａ￣（２＋）荧光指示剂技术及ＮＡＤＨ氧化偶联差光谱变化的分析方法，检测了前述培养的泡沫样细胞的胞浆Ｃａ￣（２＋）水平及膜上Ｃａ￣（２＋）依赖性ＡＴＰ酶活性。发现泡沫样细胞内的Ｃａ￣（２＋）水平为对照组细胞的２．７倍，膜上Ｃａ￣（２＋）依赖性ＡＴＰ酶活性为后者的２４％。实验结果提示，在巨噬细胞源性泡沫细胞的形成过程中，伴随着缓慢的Ｃａ￣（２＋）内流或释放，这可能与膜上Ｃａ￣（２＋）通道的持续开放及后期Ｃａ￣（２＋）依赖性ＡＴＰ酶的钝化有关。

The Change of intracellular Calcium in the Course of Macrophage-derived Foam Cell Formation

Previous study proved that it generated an in vitro model of macrophage-derived foam cell, namely, the characteristic pathological cell in the early atherosclerotic lesion to incubate C₅₇ BL/6J mouse peritoneal macrophages with 10 mg·L~(-1) oxidized low density lipoprotein for 4 days. With the Ca~(2+) fluorescent indicator technique and NADH-oxidizingcoupling-spectrum-analysis method, we determined the intracellular Ca~(2+)-ATPase of the above cultured foam-like cell. The results indicated that the Ca~(2+)]i level was 2. 7 times higher than that of control group cells, and the activity of Ca~(2+)-ATPase was 24% of that of the later cells. This supported that macrophage-derived foam cell formation is connected with the slow Ca~(2+) entry or release, which possibly derived from long-lasting openings of membranous Ca~(2+) channels and the inactivating of Ca~(2+)-ATPase at the late stage.