微柱凝胶卡式法检测孕妇免疫性抗体效价的应用分析

目的 探讨微柱凝胶卡式法在产前检测孕妇免疫性抗体效价的作用.方法 采用卡式法和试管抗人球蛋白方法平行检测180例O型孕妇(丈夫为A型95例,B型85例)IgG抗-A和抗-B效价,比较二者灵敏度的差异.结果 卡式法和试管法检测抗-A效价平均值分别为46.21和33.82,前者是后者1.37倍;抗-B平均效价平均值为47.18和35.02,前者是后者1.35倍.以IgG抗-A(抗-B)效价≥64判为阳性;卡式法和试管法检测方法测IgG抗-A阳性例数分别为45例(47.37％)、8例(8.42％),前者高于后者,差异有统计学意义(自由度=1,x2=33.23,P＜0.01);卡式法和试管法检测方法测IgG抗-B阳性例数分别为38例(44.71％)、10例(11.76％),前者高于后者,差异有统计学意义(自由度=1,x2=24.30,P＜0.01).结论 卡式法应用于产前孕妇免疫性抗体效价检测灵敏度高于试管法,且步骤简便,易于标准化,值得推广使用.

Application of microcolumn gel cartridge method in the detection of immunological antibody potency in pregnant women

Objective To explore the effect of microcolumn gel cassette method in prenatal detection of immunological antibody potency in pregnant women.Methods The anti-A and anti-B potency of 180 cases of O-type pregnant women (95 cases of their partners were A type and the other 85 cases were B type) were detected by both the card method and the test tube anti-human globulin method,and the difference in sensitivity between them were compared.Results The mean value of anti-A potency by the card method and the test tube anti-human globulin method were 46.21 and 33.82 respectively,the former was 1.37 times higher than the latter.The mean value of anti-B potency by the card method and the test tube anti-human globulin method were 47.18 and 35.02 respectively,the former was 1.35 times higher than the latter.To make IgG anti-A (anti-B) potency ≥64 to be positive,the positive cases of IgG anti-A by the card method and the test tube anti-human globulin method were 45 (47.37％) and 8 (8.42％) respectively,the former was higher than the latter,with statistically significant difference (degree of freedom =1,x2 =33.23,P＜0.01).The positive cases of IgG anti-B by the card method and the test tube anti-human globulin method were 38 (44.71％) and 10 (11.76％) respectively,the former was higher than the latter,with statistically significant difference (degrees of freedom =1,x2=24.30,P＜0.01).Conclusion The sensitivity of card method in prenatal detection of the antibody potency of pregnant women is higher than that of test tube anti-human globulin method,with the simple method,and easy to be standardized,worthy to be popularized.