部分液体通气对内毒素肺损伤肺表面活性物质系统超微结构的影响

目的:研究部分液体通气对内毒素肺损伤大鼠肺表面活性物质(pulmonarysurfactant,PS)系统超微结构的影响。方法:运用电子显微镜细胞化学方法,对内毒素肺损伤鼠PS系统进行观察及部分液体通气对其的影响。结果:内毒素肺损伤后可见PS层失去连续性,呈断裂或堆积;肺泡II型细胞内板层小体、线粒体空泡化,少数发生坏死并崩解、脱落进入肺泡腔。液体通气+肺损伤组PS系统损伤程度明显低于内毒素肺损伤组。结论:液体通气可明显改善内毒素肺损伤时PS系统的损伤。

The effect of partial liquid ventilation on the ultrastructure of pulmonary surfactant system in endotoxin-induced acute lung injury in rats

AIM: To study the effect of partial liquid ventilation on the ultrastructure of pulmonary surfactant(PS) system in endotoxin-induced acute lung injury. METHODS: The effect of partial liquid ventilation on the ultrastructure of PS system in endotoxin-induced acute lung injury was observed with electronmicroscope histochemistry. RESULTS: There was ultrastructure impairment of PS system in endotoxin-induced acute lung injury, the pulmonary surfactant layer was discontinuous, lamellar bodies and plasmosomes in type Ⅱ pneumonocytes vacuolated, and a few of them were even necrotized and disrupted into the alveolar space. The pulmonary surfactant layer was still continuous, the vacuolation of lamellar bodies and plasmosomes in type Ⅱ pneumonocytes was little with partial liquid ventilation. CONCLUSION: Partial liquid ventilation can lessen the impairment of PS system in endotoxin-induced acute lung injury in rats.