fliC基因在快速分型鉴定伤寒沙门菌和甲型副伤寒沙门菌的应用

目的:探讨沙门菌1相鞭毛蛋白抗原fliC基因在快速分型鉴定伤寒沙门菌和甲型副伤寒沙门菌的应用。方法:根据伤寒沙门菌和甲型副伤寒沙门菌1相鞭毛蛋白fliC-d和fliC-a基因以及菌体抗原rfbS基因的核酸序列,设计针对fliC-d、fliC-a及rfbS基因的3对特异性引物,采用多重PCR法进行检测。结果:实验结果显示,伤寒沙门菌和甲型副伤寒沙门菌分别在750 bp和329 bp处扩增出2条特异性目的条带,在258 bp处扩增出1条相同条带,非伤寒沙门菌株和甲型副伤寒沙门菌株均为阴性。结论:fliC基因检测可用于伤寒沙门菌和甲型副伤寒沙门菌的分型鉴定,而rfbS基因则无助于分型鉴定。

Selective amplification of fliC gene for rapid identification of Salmonella enterica serovartyphi and paratyphi A

Objective:To analyze the corresponding sequences of the fliC gene in Salmonella spp phase-1 flagellar antigens,and to study the effect for rapid detection of Salmonella enterica serovar Typhi and Paratyphi A.Methods:In accordance with the nucleotide sequence of filC-d,fliC-a and rfbS genes,three pairs of correspondingprimers were designed for identification of salmonella typhi and salmonella paratyphi A with multiplex PCR.Results:filC-d,fliC-a and rfbS were amplified at 750 bp,329 bp and 258 bp respectively from standard strains of salmonella typhi and salmonella paratyphi A.Non-typhi salmonella and salmonella paratyphi A strains were all negative.Conclusion:fliC gene detection can be applied to identification of salmonella typhi and salmonella paratyphi A,while rfbS gene was in vain.