双氢青蒿素抗日本血吸虫作用的体内实验观察

目的观察不同发育阶段日本血吸虫对双氢青蒿素的敏感性,探索双氢青蒿素抗日本血吸虫的效果。方法采用尾蚴腹部贴片法感染小鼠,每鼠感染日本血吸虫尾蚴40条;在血吸虫不同发育阶段灌服用药,于感染后50 d解剖小鼠,收集成虫,计算减虫率和减雌率。①在小鼠感染后2 h,3、5、7、10、14、18、21、28 d和35 d灌服双氢青蒿素（300 mg/kg）,观察双氢青蒿素对不同发育阶段血吸虫的作用效果。②以不同剂量双氢青蒿素分别给感染后7 d或35 d的小鼠用药,观察双氢青蒿素抗日本血吸虫作用的量-效关系。③以不同药物剂量分别在感染后第7天和第35天给药（共2次）,观察双氢青蒿素对日本血吸虫的作用效果。结果300 mg/kg双氢青蒿素一次灌服用药对7 d龄童虫和35 d龄成虫有明显杀灭作用,减虫率分别为64.81%和60.47%,减雌率分别为73.81%和90.48%。以200、300、400 mg/kg和600 mg/kg双氢青蒿素治疗感染后7 d小鼠,减虫率分别为46.84%、60.63%、59.55%和60.21%,减雌率分别为59.73%、72.29%、72.58%和76.61%;治疗感染后35 d小鼠,减虫率分别为47.23%、62.33%、76.31%和83.63%,减雌率分别为59.73%、89.36%、89.65%和93.96%;在感染后第7天和第35天共治疗2次,减虫率分别为58.16%、82.66%、83.42%和83.79%,减雌率分别为68.69%、90.43%、93.74%和94.63%。结论双氢青蒿素具有一定的抗日本血吸虫作用,对7 d童虫和35 d成虫较为敏感。

Effect of dihydroarteminisin against Schistosoma japonicum in vivo

Objective To investigate the sensitivity of dihydroartemisinin against different developmental stages of Schistosoma japonicum, so as to understand the effect of dihydroarteminisin against on S. japonicum. Methods Mice were infected with 40 S. japonicum cercariae on the abdomen. Dihydroartemisinin was given intragastrically at different developmental stages of S. japonicum , and the mice were sacrificed 50 days post-infection, the adult worms were collected, and the worm reduction rates and female worm reduction rates were calculated. ① On the 2nd h, 3rd, 5th, 7th, 10th, 14th, 18th, 21st, 28th, 35th day post-infection, the mice were administered intragastrically with dihydroartemisinin at a single dose of 300 mg/kg, and the effect of dihydroarteminisin on different developmental stages of S. japonicum were observed. ② The mice were administered with different doses of dihydroartemisinin on the 7th or 35th day post-infection, and the dose-effect was explored. ③ The mice were administered on the 7th day post-infection and re-administered on the 35th day post-infection, respectively with different doses of dihydroartemisinin, and the effect was evaluated. Results The dihydroartemisinin at a single dose of 300 mg/kg had obvious effect on 7-day schistosomula and 35-day adult worms, with 64.81% and 60.47% of worm reduction rates and 73. 81% and 90.48% of female worm reduction rates, respectively. When the mice on the 7th day post-infection were administered with 200, 300, 400 and 600 mg/kg dihydroartemisinin, the worm reduction rates were 46.84% , 60.63% , 59.55% and 60. 21% , respectively, and the female reduction rates were 59.73% , 72.29% , 72.58% and 76.61 % , respectively. While in the mice on the 35th day post-infection, the corresponding rates were 47. 23% , 62.33% , 76.31% and 83.63% , respectively, and 59. 73% , 89. 36% , 89.65% and 93.96% , respectively. When the mice were treated twice with dihydroartemisinin on the 7th day and 35th day post-infection, the worm reduction rates were 58. 16% , 82.66% ,83.42% and 83.79% , respectively, and the female worm reduction rates were 68.69% , 90.43% , 93.74% and 94.63% , respectively. Conclusions Dihydroartemisinin has effect against S. japonicum, and the 7-day schistosomulum and 35-day adult worm are sensitive to the drug.