白藜芦醇对重症急性胰腺炎急性肺损伤作用的实验研究

目的通过对大鼠重症急性胰腺炎的研究，探讨中药白藜芦醇对重症急性胰腺炎急性肺损伤的作用。方法用4％牛黄胆酸钠0．1mL／100g逆行胆胰管注射建立重症急性胰腺炎模型，对照组用等量生理盐水建模；治疗组在建模后，阴茎静脉注射0．3mL／100g白藜芦醇溶液；溶剂组在模型建立后。阴茎静脉注射0．3mL／100g吐温80溶剂。建模后3、6、12h分别取材，进行肺组织学评分、支气管肺泡灌洗液（BALF）蛋白含量、诱生型一氧化氮合酶（iNOS）活力、血小板内皮细胞粘附分子1（PECAM-1）以及转化生长因子β1（TGF-β1）表达的测定。结果模型组与治疗组形态学相比，肺组织炎性细胞浸润、肺水肿、细胞溶解坏死明显增多，肺组织iNOS在模型组3、6、12h逐渐升高，治疗组与其相比差异有统计学意义（P〈0．05），PECAM-1、TGF-β1在模型组呈强阳性表达，治疗组表达较弱。结论白藜芦醇在重症急性胰腺炎急性肺损伤中通过降低iNOS、PECAM-1、TGF-β1含量，从而减少白细胞内皮细胞粘附并降低炎性细胞局部浸润，达到治疗重症急性胰腺炎急性肺损伤的目的。

The Study in Resveratrol Function to Acute Lung Injury Sourced from Severe Acute Pancreatitis

OBJECTIVE: To study the effect of resveratrol to the onset and progress of acute lung injury in rat model of severe acute pancreatitis (SAP). METHODS: The SAP model was made by injecting 4% sodium taurocholate, a dosage of 0.1 mL/100 g, into the biliopancreatic duct. The control group is formed by injecting the equal amount of N. S into the biliopancreatic duct. For treatment group, after induction of the SAP model, the resveratrol was used to injection at a dosage of 0.3 mL/100 g through the penis vein; at solvent group, after induction of the SAP model, injecting the same amount of tween 80 through the penis vein. All rats were sacrificed at 3, 6, 12 hours. The injuries of lung were checked by pulmonary morphology change, the change of protein in bronchi alveolar lung fluid (BALF). The changes of iNOS were analyzed with special method. PECAM-1, TGF-beta1 was also detected by immunohistochemistry. RESULTS: For pulmonary morphology change, there were more inflammation cells, more pulmonary edema in experimental group than in treatment group. And in experimental group, with the postponement, the amount of iNOS is increasing (P < 0.05). However, in treatment group, the amout of iNOS was significantly less than in experimental group. The same case was that PECAM-1 and TGF-beta1 of experimental group were higher expression than that of treatment group (P < 0.05). CONCLUSION: Resveratrol can inhibit the expression of iNOS, PECAM-1, TGF-beta1, and reduce the adhesion between inflammatory cells and endothelium cells, so it may reduce the severity of acute lung injury complicated with severe acute pancreatitis.