肿瘤坏死因子、白细胞介素-2、α-干扰素及合用异博定对人乳腺癌细胞多药耐药性的逆转作用研究

目的检测肿瘤坏死因子、白细胞介素-2、α-干扰素及合用异博定对人乳腺癌耐药细胞系的耐药性的调节作用。方法MTT法检测药物对MCF-7/Adr细胞的抑制率变化，流式细胞术检测肿瘤坏死因子、白细胞介素-2、α-干扰素及合用异博定后，细胞内罗丹明-123的药物浓度变化。结果α-干扰素可增加耐药细胞内的药物浓度，α-干扰素与异博定合用比单用异博定也可增加细胞内的药物浓度。各用药组均可不同程度提高阿霉素对耐药细胞的抑制率。结论乳腺癌化疗中，合用α-干扰素及异博定，可在减少副作用的基础上逆转肿瘤的耐药性。

The study on reversal effect of multidrug resistance in human b reast cancer cells by cytokines and verapamil

Objective　To investigate the modulating effect of multidrug resistance in human breast cancer MCF-7/Adr cells by tumor necrosis factor,α-interferon,interleukin-2 and with verapamil.Methods　Chemosensitivity of cells to doxorubicin was analysed using the MTT Assay bef ore and after the medication.P-glycoprotein function of MCF-7/Adr cells treate d by verapamil was examined after accumulation of the fluorescent drug,Rhodamine -123 by flowcytometry.Results　α-interferon could increase dr ug concentration in multidurg resistant cancer cells,α-interferon with verapam il also could increase drug concentration compared with using verapamil only.All drugs could increase chemosensitivity to doxorubicin on different degrees,but t he content of drug couldn't be increased significantly by tumor necrosis factor and interleukin-2.Conclusions　α-interferon could reverse mul tidrug resistance of human breast cancer MCF-7/Adr cells.In chemotherapy of bre ast cancer,We could use α-interferon and verapamil to reverse multidurg resist ance.Verapamil had high cardiovascular toxicity,We could reduce the side effect of verapamil by using low-dose of verapamil and α-interferon.This study prov ides a new methods to reverse multidrug resistance in clinical application.