小鼠类巨噬细胞系超微结构的观察

本文报道了小鼠类巨噬细胞系(MMC-1)的超微结构。在透射电镜下,可见暗细胞、亮细胞及空细胞。这三种细胞有一定的比例。在其他培养细胞中也见到了类似的三种细胞。暗细胞电子致密度最高,呈圆形、椭圆形或梭形。表面有许多突起,有的呈丘状。胞质可分内、外两部分。外胞质无细胞器;内胞质中有丰富的线粒体、粗面内质网和聚核蛋白体。溶酶体易见,高尔基复合器发达,有成堆的微纤维。胞核不规则,多呈肾形,位于细胞的一侧。核膜清楚,常染色质较多。有的胞核中可见核仁。亮细胞突起少,线粒体肿胀,溶酶体与脂滴易见,核肿胀。空细胞的突起更少,内质网明显减少或消失,溶酶体多,次级溶酶体易见胞核大,染色质稀疏。本文根据此细胞的超微结构特点,证实其为巨噬细胞,并根据其来源称之为类巨噬细胞。

FINE STRUCTURE OF MMC-1 MOUSE MACROPHAGE-LIKE CELL LINE

The fine structure of MMC-1 mouse macrophage-like cells was studied by means of electron microscopy. Electron micrographs of these macrophages revealed dark cells, light cells and empty cells according to their density. These three cell types could also be found in other cultural cell lines.The dark cells had the highest electron density. They were round, elliptical or spindle-like in shape and many protrusions were observed on the cell surface. The endoplasm and ectoplasm could be divided clearly. There Were no organelles in the latter. In the endoplasm mitochondria, rough endoplasmic reticulum and polyribosome were abundant, lysosomes, Golgi complexes and microfilaments were easily found. Nucleus with irregular shape located in one side of the cell, euchromatins were prominent, and nucleoli appeared in some cells. Light cells had less cytoplasmic processes, dilated mitochondria, abundant lysosomes, fat droplets and dilated nuclei. Empty cells had only a little cytoplasmic processes, less endoplasmic reticulum and a lot of primary and secondary lysosomes. Sparse chromatins were distributed in the dilated nucleus.According to these fine structures and the origin of this cell line the name of the macrophage-like cell line was suggested.