| 微小RNA miR-199a／a^＊模拟物转染肝癌细胞HepG2中Met原癌基因的表达 |
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| 引用本文： | 张烈民. 微小RNA miR-199a／a^＊模拟物转染肝癌细胞HepG2中Met原癌基因的表达[J]. 胃肠病学和肝病学杂志, 2009, 18(8): 714-716 |
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| 作者姓名： | 张烈民 |
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| 作者单位： | 湖北省妇幼保健院内科,湖北,武汉,430070 |
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| 摘 要： | 目的探讨在肝癌细胞系HepG2中，Met是否为miR-199a／a^＊的靶基因。方法将miR-199a／a^＊的模拟物及阴性对照物分别转染至HepG2细胞中，转染后48h及72h分别提取RNA及蛋白行RT—PCR及Western Blot检测Met的表达。结果与阴性对照相比，转染Up-miR-199a^＊组的MetmRNA（P〈0．05）和蛋白（P〈0．001）表达水平均显著下调；而Up—miR-199a组与阴性对照组相比无统计学差异。结论肝癌细胞HepG2中miR-199a^＊负调控Met的mRNA及蛋白表达。
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| 关 键 词： | 微小RNA miR-199a/a^＊ Met 肝癌 HepG2细胞系 |
The expression of oncogene Met in the miR-199a/a* mimic transfected HepG2 cell line |
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| ZHANG Liemin. The expression of oncogene Met in the miR-199a/a* mimic transfected HepG2 cell line[J]. Chinese Journal of Gastroenterology and Hepatology, 2009, 18(8): 714-716 |
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| Authors: | ZHANG Liemin |
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| Affiliation: | ZHANG Liemin( Department of Internal Medicine, Hubei Women and Children＇ s Hospital, Wuhan 430070, China) |
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| Abstract: | Objective To explore Met gene is the target of miR-199a/a^＊ or not in the hepatocellular carcinoma cell line HepG2. Methods The simulant of miR-199a/a^＊ or the negative control （NC） were transfected into HepG2 cells. Total RNA was isolated from transfected cells at 48 hours post-transfection and protein at 72 hours. Then Met expression was detected by RT-PCR and Western Blot. Results Compared with NC group, the expressions of Met mRNA （P 〈 0.05 ） and protein （P 〈0. 001 ） were obviously down-regulated in Up-miR-199a^＊ group. However, there was no significant difference between Up-miR-199a and NC groups. Conclusion In HepG2 cell line, miR-199a^＊ negatively regulate Met expression both in mRNA and protein level. |
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| Keywords: | miR-199a/a* Met |
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