Monoclonal antibodies to HLA recognize monomorphic and polymorphic epitopes on BoLA |
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| Authors: | PATRICK CHARDON JORGE KALIL HUBERT LEVEZIEL JACQUES COLOMBANI MARCEL VAIMAN |
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| Affiliation: | Laboratoire de Radiobiologie Appliquée, Jouy en Josas, France;Laboratoire d'Immunogénétique de la Transplantation Humaine, Hôpital Saint-Louis, Paris, France;Laboratoire de Génétique Biochimique, Jouy en Josas, France;Laboratoire de Radiobiologie Appliquée, Département de Protection, Jouy en Josas, France |
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| Abstract: | Fourteen monoclonal antibodies recognizing monomorphic and polymorphic epitopes on class I and class II antigens of the human MHC have been assayed on lymphocytes of a panel of 20–150 BoLA typed bovine animals from 12 different breeds. Some monomorphic antibodies cross-reacted and others did not. Two polymorphic monoclonal antibodies in man recognize a polymorphism in cows that follows allospecificities (BoLA-w3, w9) already described. Immunoprecipitation experiments with monomorphic anti-B2m and anti-HLA-DR monoclonal antibodies have shown that these cross-reactions concern BoLA antigens. They also revealed that Ia-like antigens in cattle present the same two chain features characterized in other species. |
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