微量元素锌、铜、硒对单个核细胞产生肿瘤坏死因子的影响

本文采用L929细胞毒试验,在体外观察了三种微量元素锌、铜、硒对正常人外周血单个核细胞产生肿瘤坏死因子(TNF)的影响。结果发现锌、铜在体外能明显促进TNF产生,以0.25mmol/L为最适浓度,并能维持96小时以上。铜的作用较锌为强。硒虽然单独不能诱导TNF分泌,但与LPS协同能促使单个核细胞释放TNF。本文结果对研究上述三种元素参与免疫反应调节机理及TNF分泌调控均有裨益。

EFFECTS OF TRACE ELEMENTS ZINC,COPPER AND SELENIUM ON TUMOR NECORSIS FACTOR PRODUCTION

In this report we employed L 929 cell lytic assay for tumor necrosis factor (TNF) to observe the effects of three trace elements(Zinc, Copper and Selenium) on TNF production in normal human peripheral blood mononuclear cells (PBMC). The results showed that zinc and copper were able to augment TNF production in PBMO in vitro. The most optimal concentration of zinc and copper was 0.25 namol/L. They were both able to maintain TNF secretion for as long as 96 hours. The effect of copper was stronger than that of zinc. The results also showed that selenium alone had no effect on TNF production. However, when lipopolysacharide (LPS) was added, they were able to enhance TNF secretion. The present study strongly suggested that zinc, copper and selenium might play an important role in regulating TNF production and their immune regulation function may be mediated by TNF releases.