滇白珠抗风湿性关节炎活性成分研究

目的:对滇白珠的各提取液进行抗大鼠佐剂性关节炎活性筛选,为临床应用滇白珠治疗风湿性关节炎提供实验基础.方法:制备滇白珠的水提取总液、乙醇提取总液、醋酸乙酯提取液、正丁醇提取液、乙醇提取总液剩余部分液.滇白珠的正丁醇部分,在大孔树脂中用不同溶液洗脱,得到水洗脱部分、30%乙醇洗脱部分和95%,乙醇洗脱部分.采用大鼠佐剂性关节炎模型,观察滇白珠不同部分提取液对大鼠关节肿胀的影响.结果:滇白珠的水提液组(A)、酸酸乙酯提取液组(C)和正丁醇提取液组(D)可明显减轻大鼠关节肿胀程度,正丁醇提取液组(D)作用最强(P<0.05,P<0.01).进一步研究显示,滇白珠正丁醇提取液中水洗脱液和30%乙醇洗脱液均有抗大鼠佐剂性关节炎作用,而30%,乙醇洗脱液作用更明显(P<0.05,P<0.01).结论:滇白珠正丁醇提取液30%乙醇洗脱部分有明显的抗大鼠佐剂性关节炎作用.

Effects of Gaultheria yunnanensis on adjuvant arthritis in rats

Objective: Gaultheria yunnanensis (Franch.) Rehd. are used widespreadly in the south of China to treat rheumatoid arthritis. The aim of this study was to provide an experimental basis for G. yunnanensis to therapy rheumatoid arthritis. Method: We prepared water extracts, ethanol extracts, n-butanol extracts, ethyl acetate extracts and the rest of ethanol extracts from G. yunnanensis. Then, the n-butanol extracts were applied to macroporous resin and eluted with water, 30% ethanol, and 95% ethanol. Rheumatoid arthritis was induced by Freund's complete adjuvant injected into right postpedes in Wistar rats which was utilized to elucidate the anti-inflammatory effect of different extracted liquid of G. yunnanensis. Rats intragastric injected (i.g.) with extracts as experimental group or normal sodium as control group. Result: Freund's complete adjuvant induced arthritis was successfully established: paw edema were increased after Freund's complete adjuvant injection, peaked at 2 or 3 day, then decreased, the paw edema were increased again at 7 or 8 day, and persisted 15 d. Data show that rats were administered with water extracts, n-butanol extracts or ethyl acetate extracts produced a significant decrease paw edema which induced by Freund's complete adjuvant as compared with the control group (P<0.05, P<0.01). The effect of n-butanol extracts was the most powerful, which began at 1 d and persisted 15 d after intragastric injection. Further, n-butanol extracts eluant with water and 30% ethanol decreased the Freund's complete adjuvant induced paw edema. The activity of extracts eluant with 30% ethanol was stronger than eluant with water. Conclusion: G. yunnanensis displayed considerable effects against Freund's complete adjuvant induced arthritis in rats, which is in concordance with clinical practice. N-butanol extracts and both of the eluants with water and 30% ethanol produced a significant decrease paw edema. 30% ethanol eluants show a stronger activity than others. The effects against rheumatoid arthritis of different parts of G. yunnanensis differed in degree. It is deserved to explore the potential mechanisms of anti-inflammtion of the G. yunnanensis, especially the n-butanol extracts eluant with 30% ethanol.