Clonal CD5-positive B lymphocytes in myelodysplastic syndrome with systemic vasculitis and trisomy 8 |
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Authors: | R Billström B Johansson B Strömbeck W El-Rifai M Larramendy T Olofsson F Mitelman S Knuutila |
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Institution: | (1) Divison of Haematology, Department of Medicine, University Hospital, S-22185 Lund, Sweden, SE;(2) Department of Clinical Genetic, University Hospital, Lund, Sweden, SE;(3) Department of Medical Genetics, University of Helsinki, Helsinki, Finland, FI |
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Abstract: | Bone marrow and peripheral blood from a myelodysplastic syndrome (MDS) patient with trisomy 8 and associated systemic vasculitis
was investigated for clonal lymphoid lineage involvement using simultaneous metaphase and interphase fluorescence in situ
hybridization (FISH) and immunocytochemistry with antibodies against CD13 (granulocytic), glycophorin A (GPA, erythroid),
and the lymphocytic antigens CD3, CD5, CD20, and CD22. Trisomy 8 was detected in 55% of CD13+, 40% of GPA+, 6% of CD5+, and
5% of CD20/22+, but not in CD3+ cells. In a complementary experiment using interphase FISH on bone marrow cells sorted by
flow cytometry, 13% of CD5/CD19 double-positive cells (76% purity) were found to be trisomic. The results indicate the existence
of a small CD5-positive B-lymphoid clone as part of the MDS process in this patient. Since CD5/19-positive cells have been
proposed to be autoantibody producing, this finding might be a clue to the pathogenesis underlying the propensity for MDS
patients to develop immune-mediated complications.
Received: 2 September 1996 / Accepted: 17 October 1996 |
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Keywords: | MDS Vasculitis Lymphoid cells Trisomy 8 CD5/CD19 |
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