| 酿酒酵母中的D-(-)-扁桃酸脱氢酶 |
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| 引用本文： | 严芬,郭养浩,林木仙,刘盈盈. 酿酒酵母中的D-(-)-扁桃酸脱氢酶[J]. 药物生物技术, 2007, 14(1): 43-47 |
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| 作者姓名： | 严芬 郭养浩 林木仙 刘盈盈 |
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| 作者单位： | 福州大学药物生物技术和工程研究所,福建,福州,350002 |
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| 摘 要： | 酿酒酵母BY1．1b对苯乙酮酸不对称还原成D-（-）-扁桃酸的反应具有高度的催化还原活性和光学选择性。采用超声波细胞破碎、硫酸铵沉淀、DEAE-Sepharose FF离子交换层析及Sephacryl S-200分子筛层析等步骤，从酵母BY1．1b中分离纯化得到D-（-）-扁桃酸脱氢酶。该酶的相对分子质量约60000。该酶的生物合成模式为非细胞生长偶联型和非底物诱导型。该酶与酵母醇脱氢酶、马肝醇脱氢酶、乳酸脱氢酶及丙酮酸脱氢酶的底物特异性具有显著差别，是一种新型的脱氢酶。
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| 关 键 词： | 酿酒酵母 D-(-)-扁桃酸脱氢酶 纯化 底物特异性 |
| 文章编号： | 1005-8915（2007）01-0043-05 |
| 修稿时间： | 2006-08-182006-11-08 |
D -(-)-Mandelate Dehydrogenase from Saccharomyces Cerevisiae |
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| YAN Fen,GUO Yang-hao,LIN Mu-xian,LIU Ying-ying. D -(-)-Mandelate Dehydrogenase from Saccharomyces Cerevisiae[J]. Pharmaceutical Biotechnology, 2007, 14(1): 43-47 |
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| Authors: | YAN Fen GUO Yang-hao LIN Mu-xian LIU Ying-ying |
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| Affiliation: | Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350002, China |
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| Abstract: | Saccharomyces cerevisiae sp. strain BY1.1b showed high bioreduction activity and optic selectivity for the asymmetric bioreduction of benzoylformate to form D-(-)-mandelate (D-(-)-MA). D-(-)-mandelate dehydrogenase (D-(-)-MDH) was isolated and purified from BY1.1b by a combination of ultrasonic process, ammonium sulfate precipitation, DEAE-Sepharose Fast Flow anion-exchange chromatography and Sephacryl S-200 gel filtration chromatography. The molecular weight of D-(-)-MDH was 60000. The biosynthesis of enzyme is irrelative with the growth of yeast cell and non-inducement of substrate. D-(-)-MDH from Saccharomyces cerevisiae sp. strain BY1.1b is a novel dehydrogenase, which is distinct with other dehydrogenases in substrate specificity. |
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| Keywords: | Saccharomyces cerevisiae D-(-)-mandelate dehydrogenase Purification Specificity |
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