| 法医学组织病理切片STR分型影响因素的比较分析 |
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| 引用本文： | 张越,陈阳,杨元立,李继周,李朝品.法医学组织病理切片STR分型影响因素的比较分析[J].济宁医学院学报,2011(6):385-388,422. |
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| 作者姓名： | 张越 陈阳 杨元立 李继周 李朝品 |
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| 作者单位： | 皖南医学院法医学系;公安部物证鉴定中心; |
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| 基金项目： | 安徽省高校青年教师科研资助计划项目(编号:2007jq1176); 安徽省自然科学基金项目(编号:070413088); 国家自然科学基金资助项目(编号:C080804) |
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| 摘 要： | 目的 研究法医学病理切片DNA的基因型,探讨病理切片的组织类型、保存时间与尸源年龄对STR分型的影响.方法 运用16位点Loci-STRs复合荧光标记STR-PCR技术,采用7阶重复拉丁方实验设计,取14例尸检7个年龄段的新鲜组织样本,制作脑、心、肝、肾、脾、肺、肠等7类组织病理切片,将切片随机分组保存7d、14d、3...
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| 关 键 词： | 法医学 组织病理切片 STR分型 |
Comparative analysis of influential factors for STR genotyping in forensic pathological sections |
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| ZHANG Yue,LI Chao-pin,CHEN Yang,et al.Comparative analysis of influential factors for STR genotyping in forensic pathological sections[J].Jounal of Jinning Medical College,2011(6):385-388,422. |
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| Authors: | ZHANG Yue LI Chao-pin CHEN Yang |
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| Institution: | ZHANG Yue,LI Chao-pin,CHEN Yang,et al(Department of Forensic Science,Wannan Medical College,Wuhu 241002,China) |
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| Abstract: | Objective To explore the effects of tissue types,length of pathological sections preserved and death ages on STR genotyping through fluorescent 16 loci multi-STR-PCR detection. Methods Pathological sections were prepared from the brain,heart,liver,lung,spleen,kidney and ileum tissue obtained in 14 cases of fresh autopsy by 7 death ages,and preserved for 7,14,30,90,180,360 and 720 days,respectively,in terms of Latin square by double 7-order design.The DNA templates were extracted by silicon bead method,and D... |
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| Keywords: | Forensic genetics Pathological section STR genotyping |
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