| 长期高脂饲料喂养致小鼠肥胖模型的建立 |
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| 引用本文： | 崔立坤. 长期高脂饲料喂养致小鼠肥胖模型的建立[J]. 济宁医学院学报, 2011, 34(4): 240-242. DOI: 10.3969/j.issn.1000-9760.2011.04.004 |
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| 作者姓名： | 崔立坤 |
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| 作者单位： | 济宁医学院基础医学与法医学院,山东济宁,272067 |
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| 摘 要： | 目的长期高脂饲料喂养致小鼠肥胖模型并探讨其糖脂代谢的特点。方法采用高脂饲料喂养小鼠三个月,实验结束时测定血糖、血脂、肝体系数和脂体系数,实时荧光定量PCR方法检测肝脏、骨骼肌中脂代谢关键基因mRNA水平。结果长期高脂饲料喂养可升高小鼠的血糖、血脂和脂体系数;降低肝脏、骨骼肌中CPT1 mRNA的表达（P〈0.05）。结论长期高脂饲料喂养可引起小鼠的脂代谢紊乱及糖耐量的异常。
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| 关 键 词： | 高脂饲料 高脂血症 小鼠 |
Establishment of obesity mouse model by long-term high fat diet |
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| CUI Li-kun. Establishment of obesity mouse model by long-term high fat diet[J]. Jounal of Jinning Medical College, 2011, 34(4): 240-242. DOI: 10.3969/j.issn.1000-9760.2011.04.004 |
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| Authors: | CUI Li-kun |
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| Affiliation: | CUI Li-kun(Academy of Basic and Forensic Medicine,Jining Medical University,Jining 272067,China) |
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| Abstract: | Objective To establish the obesity mouse model by long-term high fat diet and explore the characteristics of glucose and lipid metabolism.Methods The mice were treated with high fat diet for three months.Their serum levels of sugar and lipid,and index of liver and fat to body weight were also measured.The quantitive analysis of mRNA expression of genes related to lipid metabolism was performed by real-time PCR.Results Compared with control group serum glucose level,serum lipid level and index of fat to body... |
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| Keywords: | High fat diet Hyperlipidemia |
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