黄芪对哮喘小鼠CD4^＋CD25^＋调节性T细胞及IL-4IFN-γIL-10的影响

目的探讨黄芪对哮喘小鼠CD4^＋CD25^＋调节性T细胞及IL-4、IFN-γI、L-10的影响。方法将BALB/c小鼠30只随机分为对照组、哮喘组和黄芪组。以卵蛋白（OVA）致敏激发法制备小鼠哮喘模型。酶联免疫吸附试验（ELISA）法检测支气管肺泡灌洗液（BALF）中IL-4、IFN-γ、IL-10及血清中IL-10的含量;流式细胞术（FCM）、反转录聚合酶链反应（RT-PCR）分别检测小鼠脾脏中CD4＋CD25＋调节性T细胞数量及FoxP3 mRNA表达情况。结果哮喘组小鼠BALF中IL-4含量明显高于对照组（P〈0.01）而低于黄芪组（P〈0.01）。与对照组相比,哮喘组小鼠BALF中IFN-γI、L-10、血清中IL-10含量及脾脏中CD4＋CD25＋调节性T细胞数量、FoxP3 mRNA表达水平明显降低（P〈0.01）;而黄芪组的上述改变较哮喘组显著增加（P〈0.05）。结论 CD4^＋CD25^＋调节性T细胞参与了支气管哮喘的发病过程,黄芪可通过上调CD4^＋CD25^＋调节性T细胞、FoxP3 mRNA的表达及增加IL-10含量减轻哮喘炎症。

Effect of Astragalus on CD4~+ CD25~+ T cells and cytokinesin the mice model of asthma

Objective To study the effect of Astragalus（AST） on CD4^＋ CD25^＋ regulatory T cells and IL-4、IFN-γ、IL-10 in the mice model of asthma.Methods 30 BALB/c mice were divided into 3 groups：control group,asthma group and AST group.Asthmatic mice models were established by ovalbumin intraperitoneal injection.The levels of IL-4、IFN-γ and IL-10 were measured by ELISA.Numbers of CD4^＋ CD25^＋ regulatory T cells were examined by flow cytometry;the mRNA expression of FoxP3 was tested by RT-PCR.Results The BALF level of IL-4 in asthma group was higher than Control group（P0.01）,and lower than AST group（P0.01）.Compared with control group,the BALF levels of IFN-γ、IL-10 and the serum level of IL-10 in asthma group,numbers of CD4^＋ CD25^＋ regulatory T cells and the mRNA expression of FoxP3 were decreased obviously（P0.01）.However,the changes of above-mentioned of AST group were increased significantly compared with asthma group（P0.05）.Conclusion Numbers of CD4^＋ CD25^＋ regulatory T cells in spleen and the level of IL-10 in BALF and serum decreased in asthmatic mice which might contribute to the pathogenesis of asthma.AST can increase the numbers of CD4^＋ CD25^＋ regulatory T cells,the mRNA expression of FoxP3 and the level of IL-10,and thus provides protective effects against asthma.