蚕沙叶绿素提取及合成脱镁叶绿酸工艺改进研究

目的探讨不同提取工艺对蚕沙中提取叶绿素及制取叶绿素衍生物脱镁叶绿酸的影响,以提高蚕沙叶绿素及叶绿素衍生物的制备量。方法单因素实验设计,以蚕沙软化时间(含水量)、不同提取溶剂为考察条件,优化蚕沙叶绿素提取工艺,并探讨浓盐酸脱镁时间对叶绿素合成脱镁叶绿酸制备率的影响。结果蚕沙软化时间2 h(含水量26%)、丙酮∶乙醇体积比1∶1做溶剂为提取叶绿素的最佳条件,提取率提高到1.43%。以蚕沙叶绿素粗品合成叶绿素衍生物脱镁叶绿酸,浓盐酸脱镁搅拌反应72 h制备率达670 mg.g 1。结论通过改良工艺,提高了蚕沙叶绿素及其衍生物脱镁叶绿酸的提取制备率。

Improvement on Extraction of Chlorophyll and Synthesis of Pheophorbide from Silkworm Excrement

OBJECTIVE To explore the influence of different extraction technologies on the extraction of chlorophyll and chlorophyll derivatives from silkworm excreta in order to improve the yield. METHODS The extraction technology of chlorophyll from silkworm excrement was optimized with softening time and different solvents using single factor experiments design. The effects of magnesium removal time with hydrochloric acid on yield of pheophorbide from chlorophyll have been investigated. RESULTS In the present study, the optimized extraction technology of chlorophyll from silkworm excrement was as follows： the softening time of silkworm excrement was 2 h （26% of water content）, and the extraction solvent was 1 ： 1 of acetone . ethanol. The production rate of chlorophyll was 1.43%. The preparation parameters of pheophorbide from chlorophyll were removing magnesium with hydrochloric acid for 72 hours. The yield of pheophorbide was 670 mg.g-1. CONLUSION The rate of production of chlorophyll and pheophorbide from silkworm excrement has been increased by improving extraction technology.