| 视交叉上核脑片与NIH/3T3成纤维细胞共培养模型的建立 |
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| 引用本文： | 左晓虹,蔡彦宁,李宁,于顺,张燕莉,陈彪.视交叉上核脑片与NIH/3T3成纤维细胞共培养模型的建立[J].首都医学院学报,2009,30(5):601-606. |
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| 作者姓名： | 左晓虹 蔡彦宁 李宁 于顺 张燕莉 陈彪 |
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| 作者单位： | 左晓虹,蔡彦宁,于顺,张燕莉,陈彪(首都医科大学宣武医院老年病研究所神经生物学研究室,神经变性病教育部重点实验室);李宁(首都医科大学宣武医院老年病研究所神经生物学研究室,神经变性病教育部重点实验室;首都师范大学生命科学学院细胞发育研究室) |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展计划(973)，国家自然科学基金 |
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| 摘 要： | 目的建立视交叉上核(suprachiasmatic nucleus,SCN)脑片与NIH/3T3成纤维细胞共培养的分子时钟诱导模型。方法选用7日龄SD大鼠,分离SCN脑片或皮层脑片,分别与NIH/3T3细胞共培养,分为SCN共培养组和对照组皮质共培养组,6d后连续24h每间隔6h收集NIH/3T3细胞,抽提细胞总RNA,实时定量RT-PCR检测时钟基因Per1mRNA水平。结果SCN共培养组Per1的表达均表现明显的节律性(P=0.001),对照组大脑皮质共培养组Per1基因表达均无明显节律性变化。结论成功建立SCN脑片与NIH3T3细胞共培养模型并诱导细胞内分子时钟的节律性表达,为研究SCN调控外周组织细胞生物节律提供有力工具。
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| 关 键 词： | 昼夜节律 表达 视交叉上核 Per1 |
| 收稿时间： | 2009-07-16 |
Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition |
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| ZUO Xiao-hong,CAI Yan-ning,LI Ning,YU Shun,ZHANG Yan-li,CHEN Biao.Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition[J].Journal of Capital University of Medical Sciences,2009,30(5):601-606. |
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| Authors: | ZUO Xiao-hong CAI Yan-ning LI Ning YU Shun ZHANG Yan-li CHEN Biao |
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| Institution: | 1. Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University; Key Laboratory of Neurodegenerative Diseases of Ministry of Education;2. Laboratory of Cell and Development, College of Life Sciences, Capital Normal University |
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| Abstract: | Objective To establish a novel co-cultures model of suprachiasmatic nucleus(SCN) slices and NIH/3T3 cells in a serum-free condition. Methods SCN or cortical slices prepared from 7-day-old male SD rats were cultured with NIH/3T3 cells for 6 d. 24 h expression profile of Per1 was examined in NIH/3T3 cells using real-time PCR. Results A significant daily oscillation of Per1 mRNA expression was observed in NIH/3T3 cells co-cultured with SCN slices. The peak time of Per1 was at CT11. No daily oscillation was found in NIH/3T3 cells induced with cortical slices. Conclusion A novel co-culture model of SCN slices and NIH/3T3 cells was established, which will facilitate the studies on the communications between SCN and peripheral tissues. |
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| Keywords: | Per1 |
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