血液光量子疗法影响热休克蛋白表达的研究

目的 探讨血光量子疗法对细胞ＨＳＰ７０表达的影响及血液光量子半法治疗作用的机理。方法 利用不同剂量紫外线（ＵＶ）照射体外培养的小鼠巨噬细胞,进行ＨＳＰ７０表达的Ｎｏｒｔｈｅｒｎ印迹分析。;并用不同剂量ＵＶ照射大鼠血液后回输进行ＨＳＰ７０合成的Ｗｅｓｔｅｒｎ印迹分析。结果 小剂量ＵＶ（３０秒钟）对照后细胞内ＨＳＰ７０ｍＲＮＡ稍有增多,增大剂量（１分钟）后其表达明显增多;ＵＶ照射血液回输可引起大鼠肺、

Effect of self- blood phototherapy on the expression of heat shock protein in cells

Objective To elucidate effect of self-blood phototherapy on the expression of HSP70 and curative mechanism of self-blood phototherapy.Methods Different doses of UV were used to irradiate cultured macrophages of mouse.The expression of HSP70mRNA was determined by Northern blotting.Irradiated blood with different doses of UV was transfused into rats.The levels of HSP70 protein in lung and liver were detected by the method of Western blotting.Results HSP70mRNA was some elevated after 30s UV irradiation,and remarkably elevated after increasing UV dose(1min).The levels of HSP70protein in lung and Liver were increased,and there were no differences between transfusing irradiated blood and unirradiated blood.Conclusion Self-blood phototherapy can cause the expression of HSP70mRNA with relation to UV dose.Transfusion of irradiated blood and unirradiated blood both could induce the production of HSP70.