Expression and localization of ossification-related factors in fracture healing: Comparative study of experimental union and non-union models |
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| Authors: | Genyoh Muro-Oka Takashi Satoh Tomoyuki Masuda Tsutomu Sakuma |
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| Affiliation: | (1) Department of Pathology, Iwate Medical Univeristy School of Medicine, 19-1 Uchimaru, 020 Morioka, Japan;(2) Department of Orthopaedic Surgery, Iwate Medical University School of Medicine, 19-1 Uchimaru, 020 Morioka, Japan;(3) Present address: Division of Pathology, Iwate Prefectural Central Hospital, 1-4-1 Ueda, 020 Morioka, Japan |
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| Abstract: | The immunohistochemical expression and localization of some ossification-related factors, including a bone growth factor (bone morphogenetic protein, BMP), non-collagenous proteins (osteocalcin, BGP; bone sialoprotein, BSP), and type II collagen, were compared in union and non-union models of fracture healing in rats. In the union model, fracture healing was seen by day 25, whereas the non-union model did not show healing even on day 32. In both models, BMP, BGP, BSP, and type II collagen were expressed mainly in chondrocytes, with proliferating cell nuclear antigen (PCNA)-positive reaction in the soft callus. In the non-union model, the expression of BMP, BGP, BSP, type II collagen, and PCNA disappeared earlier than in the union model. In fracture healing, PCNA-positive cells expressed the ossification-related factors, these being essential for ossification. |
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| Keywords: | fracture healing bone morphogenetic protein immunohistochemistry |
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