毛细管气相色谱法测定藿香正气软胶囊中百秋李醇的含量

目的建立毛细管气相色谱测定藿香正气软胶囊中百秋李醇含量的方法。方法样品经超声、离心后用毛细管气相色谱法测定。色谱条件:HP-5色谱柱(30 m×0.32 mm×0.25μm);初始温度170℃,保持3 min,以1℃/min的速度升至182℃,保持1 min,进样口温度280℃,检测器280℃,氮气流速0.6 mL/min。以正十八烷为内标,FID检测。结果百秋李醇在0.041 1~0.411 2 g/L浓度范围内,峰面积与内标峰面积之比值与浓度呈良好的线性关系(Y=2.94X+0.0128,r=0.999 2);平均回收率为98.96%,RSD=1.88%(n=6)。结论本方法准确、简便、灵敏、快速,可用于测定藿香正气软胶囊中百秋李醇的含量。

Quantitative determination of patchouli alcohol in Huoxiangzhengqi Soft-Capsules by capillary gas chromatography

Objective To establish a method of quantitative determination of patchouli alcohol in Huoxiangzhengqi Soft-Capsules by capillary gas chromatography.Methods The samples of patchouli alcohol were determined by capillary gas chromatography after ultrasound treatment and centrifugation. The chromatographic procedure was performed on the column of HP-5 (30 m×0.32 mm×0.25 μm). The initial temperature was maintained at 170 ℃ for 3 minutes, and then the temperature was raised to 182 ℃ at the speed of 1 ℃/min and maintained for 1 minute. The inlet temperature of samples was at 280 ℃, the detector temperature was at 280 ℃, the flow rate of Nitrogen was 0.6 mL/min, n-octadecane was taken as the internal standard, and FID was used for detection. Results Patchouli alcohol showed a good linear relationship at the range from 0.041 1 mg/mL to 0.411 2 mg/mL in the ratio between peak area and internal standard peak area or concentration (Y=2.94X+0.0128, r=0.999 2). The average recovery was 98.96% (RSD=1.88%, n=6).Conclusion The method is accurate, easy and sensitive, which can be used for the quantitative determination of patchouli alcohol in Huoxiangzhengqi Soft-Capsules.