| 饲养层与干细胞的体外培养 |
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| 引用本文： | 岑妍慧,蓝丽霞. 饲养层与干细胞的体外培养[J]. 医学综述, 2011, 17(9) |
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| 作者姓名： | 岑妍慧 蓝丽霞 |
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| 作者单位： | 广西中医学院组织学与胚胎学教研室,南宁,530001 |
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| 基金项目： | 广西自然科学基金青年资金项目，广西教育厅科研项目，广西中医学院院级课题资助 |
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| 摘 要： | 干细胞的体外培养要求在保持其未分化状态的同时,还要保持其旺盛的分裂增殖能力,所以在原代或初期培养时,干细胞的体外培养依赖饲养层细胞及其分泌的生长因子,饲养层细胞一方面可促进胚胎干细胞的附着,另一方面可分泌一些促干细胞增殖并抑制其分化的细胞因子,不同实验室在体外培养干细胞过程中所使用的饲养层细胞有所不同,故在此予以综述。
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| 关 键 词： | 饲养层 干细胞 体外培养 |
Feeder and Stem Cells in Vitro |
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| CEN Yan-hui,LAN Li-xia. Feeder and Stem Cells in Vitro[J]. Medical Recapitulate, 2011, 17(9) |
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| Authors: | CEN Yan-hui LAN Li-xia |
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| Affiliation: | CEN Yan-hui,LAN Li-xia,HE Guo-zhen.(Department of Histology and Embryology,Guangxi Traditional Chinese Medical University,Nanning 530001,China) |
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| Abstract: | The culture of stem cells in vitro requires the maintenance of stem cell undifferentiated state and potent proliferative capacity.Therefore,primary or initial culture of stem cells in vitro depends on feeder cells and growth factors secreted by stem cells.Feeder cells can promote the attachment of embryonic stem cells and simultaneously release some cytokines that enhance stem cell proliferation but suppress differentiation.The feeder cells used for the culture of stem cells in vitro vary greatly among labo... |
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| Keywords: | Feeder layer Stem cells Culture in vitro |
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