恒温扩增技术在分子诊断中的应用

聚合酶链反应(PCR)技术是近年来广泛应用于科学研究和临床检验的一项技术。目前传统的PCR技术中核酸的扩增存在效率不高且需要专用的仪器设备等缺陷,而恒温扩增技术由于引物种类多样,检测方法多变,从而克服了传统PCR核酸扩增技术的缺点,使其越来越多地被应用于分子诊断中。经国内外学者的研究证实,恒温扩增技术有着广泛的应用前景,现着重对恒温扩增技术予以综述。

Application of Isothermal Amplification Technology in Molecular Diagnosis

Polymerase chain reaction(PCR) is a technology which is widely applied in scientific research and clinical examination.At present,the conventional PCR employed by nucleic acid amplification has some shortcomings,such as low efficiency,needing of special instrument,etc.On the contrary,the isothermal amplification methods have overcome these disadvantages by utilizing a rich variety of primers and testing methods.It is confirmed by domestic and oversea scholars that a wide range of medical applications of iso...