| 高效液相色谱法测定川东獐牙菜中獐牙菜苦苷的含量 |
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| 引用本文： | 蔡兴东,杨建平,黄春,沈力,李润琴,刘丹,张乐星. 高效液相色谱法测定川东獐牙菜中獐牙菜苦苷的含量[J]. 中国医药导报, 2011, 8(5): 42-43 |
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| 作者姓名： | 蔡兴东 杨建平 黄春 沈力 李润琴 刘丹 张乐星 |
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| 作者单位： | 重庆三峡医药高等专科学校,重庆万州,404020 |
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| 基金项目： | 重庆市教育委员会科学技术研究项目资助 |
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| 摘 要： | 目的：建立川东獐牙菜中獐牙菜苦苷的含量测定方法。方法：采用高效液相色谱法,Crest C18（4.6 mm×250 mm,5μm）色谱柱,流动相：甲醇-水（25∶75）,流速：1 ml/min,检测波长：254 nm,柱温：25℃。结果：獐牙菜苦苷在0.4～2.0μg范围内线性关系良好（r=0.999 9）,平均回收率为99.78%,RSD=1.86%（n=6）。结论：该方法简便、准确,重现性好,可作为川东獐牙菜中獐牙菜苦苷的含量测定方法。
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| 关 键 词： | 川东獐牙菜 獐牙菜苦苷 含量测定 |
Determination of Swertiamarin in Swertia davidi Franch.by HPLC |
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| CAI Xingdong,YANG Jianping,HUANG Chun,SHEN Li,LI Runqin,LIU Dan,ZHANG Lexing. Determination of Swertiamarin in Swertia davidi Franch.by HPLC[J]. China Medical Herald, 2011, 8(5): 42-43 |
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| Authors: | CAI Xingdong YANG Jianping HUANG Chun SHEN Li LI Runqin LIU Dan ZHANG Lexing |
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| Affiliation: | CAI Xingdong,YANG Jianping,HUANG Chun,SHEN Li,LI Runqin,LIU Dan,ZHANG Lexing(Chongqing Three Gorges Medicine School,Wanzhou 404020,China) |
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| Abstract: | Objective： To establish a method for the determination of Swer-tiamarin in Swertia davidi Franch.by HPLC.Methods： High performance liquid chromatography was performed on C18 column.The chromatographic conditions were as follows： methyl alcohol-water（25∶75） as mobile phase,flow rate being 1.0 ml/min,the detecting wavelength at 254 nm and column temperature being 25℃.Results： Swertiamarin had good linear relation within the range from 0.4 to 2.0 μg（r=0.999 9）.The average recovery was 99.78% with a RSD of 1.86%（n=6）.Conclusion： This method is convenient,accurate,reproducible,and can be used for content determinaton of Swertiamarin in Swertia davidi Franch.. |
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| Keywords: | Swertia davidi Franch. Swertiamarin Content determination |
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