顺铂致豚鼠耳毒性作用中细胞凋亡及caspase-3表达的研究

目的：通过顺铂（CDDP）致豚鼠耳毒性作用中细胞凋亡及caspase-3表达的观察,探讨CDDP致耳毒性的作用机制。方法：实验组和对照组豚鼠各10只,前者以CDDP 2.0 mg/（kg.d）连续腹腔注射6 d,后者以等量生理盐水替代。通过ABR测试用药前后豚鼠听力的变化,应用TUNEL法检测耳蜗凋亡细胞,免疫组化法检测caspase-3在耳蜗中的表达。结果：实验组耳蜗凋亡细胞明显增多,caspase-3的表达亦明显增强。结论：caspase-3介导的信号传导机制,可能参与了CDDP所致的耳蜗细胞凋亡过程,导致耳毒性的发生。

Study on expression of apoptosis and caspase-3 in cisplatin-induced ototoxicity of guinea pig

Objective： To explore the ototoxicity mechanism of cisplatin by investigating the expression of apoptosis and caspase-3 in ototoxicity induced by cisplatin in guinea pig.Methods： 20 guinea pigs were divided into control group（10 guinea pigs） and experiment group（10 guinea pigs）.10 guinea pigs were treated with cisplatin [2.0 mg/（kg·d）,ip,all 6 days） in the experiment group,other 10 guinea pigs were replaced with normal saline in the control group.Guinea pigs were treated with ABR testing in hearing changes before and after the treatment;expression of apoptosis were detected by TUNEL assay and immunohistochemistry method was employed to detect the caspase-3 expression in cochlear cells.Results： The apoptosis of cochlea cells increased and the espression of caspase-3 also enhanced significantly in the experiment group.Conclusion： The signal transducion mechanisms mediated by caspase-3 may be involved in CDDP-induced apoptosis of cochlear cells,leading to the occurrence of ototoxicity.