| 实时荧光定量-PCR和酶联免疫吸附法检测巨细胞病毒-IgM抗体的比较 |
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| 引用本文： | 范文勇. 实时荧光定量-PCR和酶联免疫吸附法检测巨细胞病毒-IgM抗体的比较[J]. 中国医药导报, 2011, 8(2): 83-84 |
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| 作者姓名： | 范文勇 |
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| 作者单位： | 广东省深圳市宝安区人民医院,广东深圳,518101 |
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| 摘 要： | 目的：比较实时荧光定量-PCR和酶联免疫吸附法检测巨细胞病毒-IgM抗体水平的敏感性及准确率。方法：收集108例孕前筛查妇女,同时采用实时荧光定量-PCR和酶联免疫吸附法检测外周血巨细胞病毒-IgM抗体水平,并对这两种检测方法的阳性率进行比较。结果：实时荧光定量-PCR法阳性率为74%,酶联免疫吸附法为57%,两者差异有统计学意义（P〈0.05）;18例荧光定量-PCR法阳性的妇女血清巨细胞病毒-IgM拷贝数低于10-4/ml。结论：实时荧光定量-PCR与酶联免疫吸附法相比,对巨细胞病毒-IgM抗体检测具有较高的敏感性和准确率,值得临床推广使用。
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| 关 键 词： | 实时荧光定量-PCR 酶联免疫吸附法 巨细胞病毒-IgM抗体 |
Comparison of cytomegalovirus-IgM detection by real time-PCR and enzyme linked immunoabsorption assay |
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| FAN Wenyong. Comparison of cytomegalovirus-IgM detection by real time-PCR and enzyme linked immunoabsorption assay[J]. China Medical Herald, 2011, 8(2): 83-84 |
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| Authors: | FAN Wenyong |
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| Affiliation: | FAN Wenyong(People's Hospital of Bao'an District of Shenzhen City,Guangdong Province,Shenzhen 518101,China) |
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| Abstract: | Objective: To compare the sensitivity and accuracy of cytomegalovirus-IgM(CMV-IgM) detection by real-time PCR and enzyme linked immunoabsorption assay(ELISA).Methods: We enrolled 108 females who were underwent pre-pregnancy evaluation and evaluated peripheral blood CMV-IgM level by both real time-PCR and ELISA.Positive rates of these two methods were compared.Results: Positive rate in real time-PCR was 74% while in ELISA was 57%,and there was a significant difference between these two methods(P<0.05).Copy n... |
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| Keywords: | Real-time PCR Enzyme linked immunoabsorption assay Cytomegalovirus-IgM |
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