人参皂苷Rg1对局灶性脑缺血再灌注大鼠的神经保护作用

目的:探讨人参皂甙Rg1对局灶性脑缺血再灌注(FCIR)大鼠的神经保护作用。方法:72只大鼠随机分成假手术组、对照组、尼莫地平组(10.8 mg/kg)、人参皂苷Rg1低剂量组(10 mg/kg)、人参皂苷Rg1中剂量组(20 mg/kg)和人参皂苷Rg1高剂量组(40 mg/kg),每组12只。采用大脑中动脉栓塞法制备大鼠FCIR模型。假手术组和对照组给予生理盐水,其它各组则用相应药物连续灌胃14 d。治疗结束后,评定大鼠神经功能,检测顶颞叶皮质金属蛋白酶抑制因子(TIMP1)阳性细胞数及神经细胞凋亡。结果:人参皂苷Rg1各剂量组及尼莫地平组大鼠的神经功能评分明显低于对照组(P0.05);对照组顶颞叶皮质TIMP1阳性细胞数明显高于假手术组(P0.01),人参皂苷Rg1各组和尼莫地平组TIMP1阳性细胞数明显高于对照组(P0.05或0.01);对照组顶颞叶皮质细胞凋亡率高于假手术组(P0.01),人参皂苷Rg1各组和尼莫地平组细胞凋亡率明显低于对照组(P0.05或0.01)。结论:人参皂苷Rg1可能通过促进TIMP1表达,降低神经细胞凋亡率,进而对FCIR大鼠起到神经保护作用。

Neuroprotective Effect of Ginsenoside Rg1 on Focal Cerebral Ischemia-Reperfusion Rats

Objective: To explore the neuroprotective effect of Ginsenoside Rg1 on focal cerebral ischemia-reper-fusion (FCIR) rats. Methods: Totally 72 rats were randomly divided into 6 groups: sham-operation group, control group, Nimodipine group (10.8 mg/kg), Ginsenoside Rg1 low dose group (10 mg/kg), Ginsenoside Rg1 medium dose group (20 mg/kg), and Ginsenoside Rg1 high dose group (40 mg/kg), with 12 rats in each group. The FCIR model was performed with the method of middle cerebral artery occlusion. The sham-operation and control groups were given normal saline, while other groups received corresponding medication by intragastric administration for 14 days. After the treatment, the neural function was assessed, and numbers of positive cells representing tissue in-hibitors of metalloproteinases1 (TIMP1) and ratio of cell apoptosis in the parietal and temporal cortex were detect-ed. Results: Compared with the control group, scores of neural function were decreased remarkably in all Gin-senoside Rg1 groups and Nimodipine group(P<0.05). Numbers of TIMP1-positive cells in the parietal and temporal cortex of the control group was significantly higher than that in the sham-operation group (P<0.01). Compared with the control group, numbers of TIMP1-positive cells increased remarkably in all theGinsenoside Rg1 groups and Ni-modipine group (P<0.05 or <0.01). Ratio of cell apoptosis in parietal and temporal cortex of the control group was significantly higher than that in the sham-operation group (P<0.01). Compared with the control group, ratio of cell apoptosis was decreased remarkably in all the Ginsenoside Rg1 groups and Nimodipine group (P<0.05 or <0.01). Conclusion: Ginsenoside Rg1 may promote the expression of TIMP1, thus decrease the ratio of cell apoptosis and finally improve the neural function in FCIR rats.