大蒜素对人皮肤基底细胞癌A431细胞的放疗增敏作用研究

目的 探讨大蒜素(Allicin)是否能增加人皮肤基底细胞癌A431细胞的放疗敏感性及其作用机制.方法 MTT法检测Allicin对A431细胞的生长抑制率,筛选出半数抑制浓度(IC50);将细胞分为对照组、照射组(IR组)、Allicin组和IR+Allicin组.采用流式细胞仪检细胞自噬水平.结果 MTT结果显示大蒜素作用A431细胞24、48、72 h后IC50分别为35.47、18.64、6.56 mmol/L(F =22.54、18.94和21.63,P＜0.05).和对照组相比,Allicin组和Allicin+ IR组A431细胞的自噬水平均增加(F=30.15、28.36,P＜0.05),以Allicin+ IR组升高最显著.结论 Allicin通过上调人皮肤基底细胞癌A431细胞的自噬水平来增加其放疗敏感性.

Radiosensitization effect of allicin on human skin basal cell carcinoma A431 cell

Objective To investigate radiosensitization effect of Allicin on human skin basal cell carcinoma A431 cell.Method MTT was used to detect IC50.Cells were divided into control,ionizing radiation group (IR),allicin group,IR + allicin group.Flow cytometry was used to analyze the changes of autophagy level.Results MTT showed that,after co-cultured with allicin,the IC50 of allicin was 35.47,18.64,6.56 mmol/L at 24h,48h and 72h,respectively (F =22.54,18.94 and 21.63,P ＜ 0.05).Compared with control,allicin group and IR + allicin group showed large1y increased autophagy in A431 cell(F =30.15 and 28.36,P ＜ 0.05),especially in IR + allicin group.Conclusion Allicin could increase the radiosensitivity of human skin basal cell carcinoma A431 cell which was associated with autophagy.