青蒿琥酯对MRL/lpr小鼠间质性肺炎和颌下腺炎的治疗作用

目的 探讨青蒿琥酯对MRL/lpr小鼠间质性肺炎和颌下腺炎的治疗作用.方法 18只MRL/lpr小鼠按随机数字表法分为硫酸羟氯喹组、青蒿琥酯组和对照组,每组6只.18周龄时,硫酸羟氯喹组和青蒿琥酯组分别给予硫酸羟氯喹混悬液(150 mg/kg,1次/d)和青蒿琥酯混悬液(50 mg/kg,1次/d),共12周.HE染色法评价肺炎和颌下腺炎的病理学改变.采用酶联免疫吸附法检测血清和尿单核细胞趋化蛋白-1 (monocyte chemoattractant protein-1,MCP-1)水平.结果 30周龄时,硫酸羟氯喹组、青蒿琥酯组细支气管周围损害指数[(1.62±0.19)、(1.52±0.30)比(1.95±0.34);P＜0.05]、血管周围损害指数[(1.23±0.18)、(1.28±0.12)比(1.57±0.33);P＜0.05]、肺泡损害指数[(1.35±0.16)、(1.37±0.10)比(1.72±0.34);P＜0.05]、颌下腺炎症指数[(1.48±0.22)、(1.43±0.15)比(1.84±0.34);P＜0.05]均较对照组减轻,血清[(1 103.02±185.56) pg/ml、(1 072.37±242.43) pg/ml比(1 490.67±329.43) pg/ml;P＜0.05]和尿[(189.16±70.85) pg/ml、(198.79±113.47) pg/ml比(446.79±192.31) pg/ml;P＜ 0.05] MCP-1水平均显著降低.结论 青蒿琥酯可降低MRL/lpr小鼠血清MCP-1水平,减轻间质性肺炎和下颌腺炎.

Effects of artesunate on interstitial pneumonia and submaxillaritis in MRL/lpr mice

Objective To investigate the effects of artesunate (ART) on interstitial pneumonia and sialadenitis in MRL/lpr mice.Methods A total of 18 MRL/lpr mice were randomly allocated to a hydroxychloroquine sulfate (HCQ) group,a ART group and a control group.At the age of 18 weeks,the mice in the HCQ group and ART group were given HCQ 150 mg/kg daily and ART 50 mg/kg daily for 12 weeks,respectively.The histopathological changes of pneumonitis and submaxillaritis were assessed by hematoxylin and eosin staining.The levels of monocyte chemoattractant protein-1 (MCP-1) in the serum and urine were measured by the enzyme-linked immunosorbent assay.Results At the age of 30 weeks,the index of peribronchiolar lesion (1.62 ± 0.19,1.52 ± 0.30 vs.1.95 ± 0.34;all P＜0.05),the index of perivascular lesion (1.23 ± 0.18,1.28 ± 0.12 vs.1.57 ± 0.33;all P＜0.05),the alveolar lesions index (1.35 ± 0.16,1.05 ± 0.15 vs.1.72 ± 0.34;all P＜0.05) and the submaxillaritis index (1.48 ± 0.22,1.43 ± 0.15 vs.1.84 ± 0.34;all P＜0.05) in the HCQ group and the ART group were significantly decreased than those in the control group.The MCP-1 levels in the serum (1 103.02 ± 185.56 pg/ml,1 072.37 ± 242.43 pg/ml vs.1 490.67 ± 329.43 pg/ml;all P＜0.05) and urine (189.16 ± 70.85 pg/ml,198.79 ± 113.47 pg/ml vs.446.79 ± 192.31 pg/ml;all P＜0.05) in the HCQ group and the ART group were significantly lower than those in the control group.Conclusion ART can decrease the MCP-1 level,and ameliorate interstitial pneumonitis and sialadenitis in MRL/lpr mice.