氨茶碱对移植物抗宿主反应及同种心脏移植物存活时间的影响

本文报告氨茶碱对移植物抗宿主反应及同种异位心脏移植物存活时间的影响。结果表明,人类T细胞在体外对氨茶碱共同温育后可分为茶碱抵抗(TR)和茶碱敏感(TS)两种,它们可分别增强和抑制总T细胞所引起的移植物抗宿主反应(GVHR)。给小鼠胃饲氨茶碱,其脾脏淋巴细胞所引起的GVHR也较胃饲生理盐水者为弱,提示氨茶碱在体内也可诱导具有免疫抑制作用的细胞。在此基础上,观察了氨茶碱对小鼠同种异位心脏移植物存活时间的影响,结果表明它可显著延长小鼠移植物的存活时间。

The effects of aminophylline on graft versus host reaction (GVHR) and the survival of heart allograft in mice

The effects of aminophylline on xenogenic graft versus host reaction and the survival of heart allograft in mice were investigated. Human T lymphocytes could be separated into two subsets when they were incubated with theophylline in vitro. One subset lost their ability to form E rosette with sheep red blood cells and was called theophylline sensitive T cell (TS). The other one maintained their ability to form E rosette was called thyeophlline resistant T cell (TR). It was shown that TS inhibited and TR enhanced GVHR induced by total T lymphocytes in cyclophosphamide treated rats. Spleen lymphocytes from mice treated with aminophyline showed significant weaker GVHR than that treated with normal saline, suggesting that aminophylline might induce suppressor cells in vivo. The effect of aminophylline on the survival of heart heterotopic allograft was then investigated and the result showed that aminophylline prolonged the survival of transplant significantly.