苏州地区轮状病毒腹泻的分子流行病学特征

目的了解苏州地区小儿轮状病毒(RV)性腹泻的发病规律和血清型特征。方法采用酶联免疫吸附实验、巢式逆转录聚合酶链式反应，对2001年9月～2003年8月期间5岁以下的腹泻住院病儿的粪标本，进行病毒抗原鉴定和血清分型。结果721份小儿腹泻粪标本，轮状病毒阳性为345例(47．85％)；年龄在7～18个月时发病率最高为239例(69．28％)；10月份～1月份是发病高峰季节，为248例(71.88％)；血清型分型以G3型199例(60．49％)为主，其次是G1型74例(22．49％)；少见的血清型G9型3例(0．90％)。在对G血清分型标本中的80份进行P血清分型，其中P[4]型29株，P[8]型11株，P[4]、[8]混合型8株。G型和P型的组合型是P[4]G3、P[8]G3、P[4]G1等29例(36．25％)为主，P[8]G1和P[4]G2为6例，仅占8．20％。出现2例(0．60％)P[4]G9少见组合型。结论苏州地区2001年9月～2003年8月间小儿轮状病毒性腹泻是以G3型流行毒株为主，全国则以G1型为主。

Molecular epidemioloic characteristics of rotavirus diarrhea in Suzhou

Objective To characterize the molecular epidemiology of rotavirus infection and the distribution of G and P serotype of rotavirus in Suzhou.Methods 721 stool samples, collected from children less than five years of age hospitalized between September 2001 to August 2003, were tested for rotavirus antigen by ELISA and G,P serotyping by MabsELISA and/or RT-PCR. Results 345(47.85%) rotavirus-positive samples were detected in 721 fecal specimens during the two years. A peak age distribution of rotavirus diarrhea was 7 to 18 months old(239/345,69.28%).Rotavirus infection peaked in winter months (between October and January of next year). Serotype G3 represented 60.49% of the G-typed samples predominating in all strains. Serotype G1 was the next most common, representing 22.49% of G-typed samples.Serotype G9 was uncommon, representing 0.9% of G-typed sample. Among 80 strains performed with P serotypes, 29 strains were P, 11 strains P, 8 strains mixed with Pand P. PG3,P G3 and PG1 were the most common combined serotypes(36.25%).PG1 and PG2 strains only had 8.20%.Rare combined serotype PG9(0.60%)was identified. Conclusion In Suzhou , rotavirus serotype G3 was prevalent strains between September 2001 to August 2003,which was different from the predominant G1 in the other regions of China.