| 他扎罗汀对Colo-16细胞体外增殖的影响及其机制的研究 |
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| 引用本文： | 魏志平,李圆,刘彦群.他扎罗汀对Colo-16细胞体外增殖的影响及其机制的研究[J].临床皮肤科杂志,2008,37(10). |
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| 作者姓名： | 魏志平 李圆 刘彦群 |
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| 作者单位： | 徐州医学院附属医院皮肤科,江苏,徐州,221002;徐州医学院附属医院皮肤科,江苏,徐州,221002;徐州医学院附属医院皮肤科,江苏,徐州,221002 |
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| 摘 要： | 目的:研究他扎罗汀对体外培养的皮肤鳞状细胞癌细胞株Colo-16细胞体外增殖的影响,并探讨其机制.方法:用不同浓度的他扎罗汀处理Colo-16细胞,四甲基偶氮唑蓝(MTT)法测定Colo-16细胞增殖活性;免疫组化染色技术及免疫印迹法分别测定Colo-16细胞Bcl-2、Bax蛋白表达水平.结果:不同浓度的他扎罗汀对Colo-16细胞体外增殖均具有抑制作用,其抑制作用呈明显的时效和量效关系(P<0.05);他扎罗汀作用Colo-16细胞后,Bcl-2蛋白表达明显下调,Bax表达明显增强.结论:他扎罗汀能抑制皮肤鳞状细胞癌Colo-16细胞的增殖,其机制与Bcl-2蛋白表达下调和Bax表达增加有关.
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| 关 键 词： | 他扎罗汀 癌 鳞状细胞 Colo-16细胞 蛋白 Bcl-2 Bax |
In vivo study of tazarotene on the proliferation of Colo-16 cell and the relevent mechanism |
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| WEI Zhi-ping,LI Yuan,LIU Yan-qun.In vivo study of tazarotene on the proliferation of Colo-16 cell and the relevent mechanism[J].Journal of Clinical Dermatology,2008,37(10). |
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| Authors: | WEI Zhi-ping LI Yuan LIU Yan-qun |
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| Institution: | Department of Dermatology;Affiliated Hospital of Xuzhou Medical College;Xuzhou 221002;China |
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| Abstract: | Objective:To investigate the influence of tazarotene on cultured cutaneous squamous cell line-Colo-16 and its possible mechanism.Methods:Colo-16 cells were treated with different concentrations of tazarotene.Cell viability was detected by MTT assay.The protein expression of Bcl-2 and Bax was detected by immunocytochemistry and Western-blotting.Results:Tazarotene significantly inhibited the proliferation in a time-and dose-dependent fashion(P < 0.05).Bcl-2 expression was signifi-cantly reduced after tazarote... |
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| Keywords: | tazarotene carcinoma squamous cell cell Colo-16 protein Bcl-2 Bax |
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