|
|||||
|
|
| 阿胶酶解物抗氧化肽的分离与质谱分析 | |
| 引用本文： | 李笑尘,闫利华,王智民,张启伟,高建萍,陈两绵,王锦玉,仝燕,张贵锋. 阿胶酶解物抗氧化肽的分离与质谱分析[J]. 中国中药杂志, 2013, 38(7): 1021-1025 |
| 作者姓名： | 李笑尘 闫利华 王智民 张启伟 高建萍 陈两绵 王锦玉 仝燕 张贵锋 |
| 作者单位： | 1. 首都医科大学中医药学院,北京,100069 2. 中国中医科学院中药研究所,北京100700;中药质量控制技术国家工程实验室,北京100700 3. 中国科学院过程工程研究所,北京,100190 4. 中国中医科学院中药研究所,北京,100700 |
| 基金项目： | 国家 "重大新药创制" 重大科技专项(2009ZX09308-003,2009ZX09301-005);国家高技术研究发展计划(863)项目(2010AA023005) |
| 摘 要： | 采用阴离子交换纤维及Sephadex G-25柱色谱法对阿胶酶解物进行分离纯化,以DPPH,ABTS法对阿胶酶解物及其不同部位进行体外抗氧化能力测定,从阿胶酶解物中筛选出体外抗氧化活性最强的部位,命名为GFC-1.GFC-1质量浓度为2.0g·L-1时对DPPH清除率为47.95％,0.40 g· L-1时对ABTS清除率为97.20％;利用LC-ESI-MS/MS结合TurboSEQUEST检索软件及Swiss-Prot数据库从GFC-1中鉴定出9个小分子肽,并从中识别出高重复核心序列GPAGPP* GPP*(P*为羟脯氨酸). |
| 关 键 词： | 阿胶 抗氧化肽 LC-ESI-MS/MS 氨基酸序列 |
| 收稿时间： | 2013-01-17 |
Isolation and mass spectrometric analysis of antioxidant peptides from enzymatic hydrolysates of roasted pills of Asini Corii Colla |
|
| LI Xiao-chen,YAN Li-hu,WANG Zhi-min,ZHANG Qi-wei,GAO Jian-ping,CHEN Liang-mian,WANG Jin-yu,TONG Yan and ZHANG Gui-feng. Isolation and mass spectrometric analysis of antioxidant peptides from enzymatic hydrolysates of roasted pills of Asini Corii Colla[J]. China Journal of Chinese Materia Medica, 2013, 38(7): 1021-1025 | |
| Authors: | LI Xiao-chen YAN Li-hu WANG Zhi-min ZHANG Qi-wei GAO Jian-ping CHEN Liang-mian WANG Jin-yu TONG Yan ZHANG Gui-feng |
| Affiliation: | School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China |
| Abstract: | A fraction named GFC-1 with high antioxidant activities in vitro was isolated from the enzymatic hydrolysates of roasted pills of Asini Corii Colla, and the peptides in this fraction were identified. The enzymatic hydrolysates were isolated and purified with anion exchange chromatography and Sephadex G-25 filtration chromatography successively. GFC-1, a fraction isolated from the hydrolysates, exhibited the highest DPPH and ABTS scavenging capacity (DPPH 47.95%at 2.0 g·L-1 and ABTS 97.20%at 0.40 g·L-1). Nine peptides from GFC-1 were identified by LC-ESI-MS/MS coupled with TurboSEQUEST search software and Swiss-Prot data base, and a high repetition core sequence GPAGPP*GPP* was also found. |
| Keywords: | Asini Corii Colla antioxidant peptides LC-ESI-MS/MS amino acid sequence |
| 本文献已被 万方数据 等数据库收录！ | |