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| 半夏毒针晶的致炎效应与巨噬细胞的相关性研究 | |
| 引用本文： | 赵腾斐,张倩,张雯,吴皓,郁红礼,王慧珠.半夏毒针晶的致炎效应与巨噬细胞的相关性研究[J].中国中药杂志,2013,38(7):1041-1045. |
| 作者姓名： | 赵腾斐 张倩 张雯 吴皓 郁红礼 王慧珠 |
| 作者单位： | 1. 南京中医药大学药学院,江苏南京,210046 2. 南京中医药大学药学院,江苏南京210046;江苏省中药炮制重点实验室,江苏南京210046;国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210046 3. 南京中医药大学药学院,江苏南京210046;南京中医药大学国家科技部规范化中药药理实验室,江苏南京210046 |
| 基金项目： | 国家自然科学基金项目(30772785,30973939);国家"重大新药创制"科技重大专项(2009ZX09308-004) |
| 摘 要： | 目的:研究半夏毒针晶刺激性毒性产生的机制.方法:采用小鼠腹腔巨噬细胞体外培养模型,以培养上清液中TNF-α,IL-1β和IL-6为指标,研究半夏毒针晶致炎的量-毒,时-毒曲线;采用扫描电镜法观察经半夏毒针晶刺激后巨噬细胞表面形态学的改变;使用巨噬细胞-中性粒细胞共培养迁移模型,考察半夏毒针晶刺激巨噬细胞对中性粒细胞迁移的影响.结果:半夏毒针晶刺激巨噬细胞,可引起TNF-α,IL-1β和IL-6含量显著升高,且具有剂量和时间依赖性;扫描电镜显示,半夏毒针晶可被巨噬细胞吞噬,细胞膜表面皱褶明显,伪足数量增多,细胞膜完整性下降,并能显著诱导中性粒细胞迁移.结论:巨噬细胞在毒针晶的致炎过程中起关键的介导作用.半夏毒针晶的毒性机制是毒针晶刺入组织,激活组织中的驻留巨噬细胞,引起吞噬、促炎细胞因子释放、中性粒细胞大量迁移,最终导致强烈的急性炎症反应. |
| 关 键 词： | 半夏毒针晶 巨噬细胞 炎症因子 扫描电镜 中性粒细胞趋化 |
| 收稿时间： | 2012/10/7 0:00:00 |
Study on inflammatory effect of toxic raphides from Pinellia ternate and its correlation with macrophages |
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| ZHAO Teng-fei,ZHANG Qian,ZHANG Wen,WU Hao,YU Hong-li and WANG Hui-zhu.Study on inflammatory effect of toxic raphides from Pinellia ternate and its correlation with macrophages[J].China Journal of Chinese Materia Medica,2013,38(7):1041-1045. | |
| Authors: | ZHAO Teng-fei ZHANG Qian ZHANG Wen WU Hao YU Hong-li and WANG Hui-zhu |
| Institution: | College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210046, China;Engineering Research Center for Standardization of Traditional Chinese Medicine Processing under State Ministry of Education, Nanjing 210046, China;College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210046, China;Engineering Research Center for Standardization of Traditional Chinese Medicine Processing under State Ministry of Education, Nanjing 210046, China;College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210046, China;Pharmacologic Laboratory for Standardization of Traditional Chinese Medicine under State Ministry of Science and Technology, Nanjing University of Chinese Medicine, Nanjing 210046, China |
| Abstract: | |
| Keywords: | toxic raphides from Pinellia ternata macrophage inflammatory cytokine scanning electron microscopy neutrophil chemotaxis |
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