| 玉屏风散抗OVA致小鼠过敏性哮喘的作用研究 |
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| 引用本文： | 王慧珠,洪敏,桂黎黎,华永庆,许惠琴.玉屏风散抗OVA致小鼠过敏性哮喘的作用研究[J].中国中药杂志,2013,38(7):1052-1055. |
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| 作者姓名： | 王慧珠 洪敏 桂黎黎 华永庆 许惠琴 |
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| 作者单位： | 南京中医药大学药学院国家科技部规范化中药药理实验室中药药效与安全性评价重点实验室,江苏南京,210029 |
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| 基金项目： | 国家自然科学基金项目(81073121);"青蓝工程"项目;国家教育部留学回国人员科研启动基金项目;江苏省教育厅省属高校自然科学重大基础研究项目(08KJA360001) |
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| 摘 要： | 目的:观察玉屏风散(YPFS)抗OVA致小鼠过敏性哮喘的作用.方法:建立OVA诱导的小鼠过敏性哮喘模型,第0,14天,分别腹腔注射20 μg OVA,自21 d时开始雾化吸入0.5％ OVA溶液20 min,连续7d.空白对照组给予同体积的生理盐水.YPFS低、中、高剂量组每天分别灌胃给予玉屏风散生药3.25,6.5,13 g·kg-1,模型组和空白对照组给予同体积生理盐水,阳性药组从雾化期开始腹腔注射地塞米松(DEX)1 mg·kg-1.最后一次OVA雾化后24h取血,进行血中嗜酸性粒细胞(Eos)计数,血清中IgE测定;收集支气管灌洗液(BALF)进行细胞计数、分类;取右侧肺组织研磨匀浆,检测细胞因子IL-4,IFN-γ,左侧肺上叶做病理组织学观察.结果:模型组血中Eos及血清中IgE显著增高,BALF中出现大量Eos,肺组织病理显示支气管浆液性渗出,小管上皮细胞脱落,管腔狭窄或闭塞,且肺泡间隔变宽增厚,支气管周围淋巴细胞浸润.肺组织匀浆中IL-4增加,IFN-γ/IL-4显著降低,YPFS各剂量组能降低血中Eos及血清中IgE含量,减少BALF中Eos,减轻上述模型的病理改变.同时降低肺组织匀浆中IL-4含量,提高IFN-γ/IL-4.结论:玉屏风散对OVA导致的过敏性哮喘有明显的抑制作用,表现为降低哮喘小鼠的血中Eos,IgE分泌,肺组织炎症细胞的浸润.同时降低肺匀浆中IL-4的水平,提高IFN-γ/IL-4,抑制Th细胞向Th2极化.
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| 关 键 词： | 玉屏风散 过敏性哮喘 Eos IgE IFN-γ/IL-4 |
| 收稿时间： | 2012/10/30 0:00:00 |
Effect of Yupingfeng San against OVA-induced allergic asthma in mice |
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| WANG Hui-zhu,HONG Min,GUI Li-li,HUA Yong-qing and XU Hui-qin.Effect of Yupingfeng San against OVA-induced allergic asthma in mice[J].China Journal of Chinese Materia Medica,2013,38(7):1052-1055. |
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| Authors: | WANG Hui-zhu HONG Min GUI Li-li HUA Yong-qing and XU Hui-qin |
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| Institution: | Pharmacologic Laboratory for Standardization of Traditional Chinese Medicine under State Ministry of Science and Technology, Key Laboratory of Efficacy and Safety Evaluation of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China;Pharmacologic Laboratory for Standardization of Traditional Chinese Medicine under State Ministry of Science and Technology, Key Laboratory of Efficacy and Safety Evaluation of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China;Pharmacologic Laboratory for Standardization of Traditional Chinese Medicine under State Ministry of Science and Technology, Key Laboratory of Efficacy and Safety Evaluation of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China;Pharmacologic Laboratory for Standardization of Traditional Chinese Medicine under State Ministry of Science and Technology, Key Laboratory of Efficacy and Safety Evaluation of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China;Pharmacologic Laboratory for Standardization of Traditional Chinese Medicine under State Ministry of Science and Technology, Key Laboratory of Efficacy and Safety Evaluation of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China |
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| Abstract: | |
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| Keywords: | Yupingfeng San allergic asthma Eos IgE IFN-γ/IL-4 |
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