| HPLC-DAD/ESI-Q-TOF-MS分析两面针中的生物碱 |
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| 引用本文： | 贾昌平,黄雪丽,李允,冯芳.HPLC-DAD/ESI-Q-TOF-MS分析两面针中的生物碱[J].中国中药杂志,2013,38(8):1198-1202. |
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| 作者姓名： | 贾昌平 黄雪丽 李允 冯芳 |
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| 作者单位： | 1. 中国药科大学药学院,江苏南京,210009
2. 中国药科大学药物质量控制与预警教育部重点实验室,江苏南京,210009 |
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| 摘 要： | 借助高效液相色谱-高分辨质谱(HPLC-DAD/ESI-Q-TOF-MS)联用技术,对两面针中的生物碱进行初步分析.采用汉邦C18色谱柱,以0.1％甲酸-乙腈和0.1％甲酸-水为流动相,梯度洗脱,经柱后分流分别经DAD检测器及ESI-Q-TOF-MS正离子模式采集.根据获得的母离子和碎片离子精确相对分子质量信息,结合文献,从两面针中鉴定分析了48个成分,其中,6个成分为新化合物.该方法分离度良好、灵敏度高,可用于两面针药材的定性分析,并有助于对该药材的质量控制及深入研究.
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| 关 键 词： | 两面针 高效液相色谱-高分辨质谱 生物碱 |
| 收稿时间： | 2012/12/24 0:00:00 |
Analysis of alkaloids in Zanthoxylum nitidum by HPLC-DAD/ESI-Q-TOF-MS |
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| JIA Chang-ping,HUANG Xue-li,LI Yun and FENG Fang.Analysis of alkaloids in Zanthoxylum nitidum by HPLC-DAD/ESI-Q-TOF-MS[J].China Journal of Chinese Materia Medica,2013,38(8):1198-1202. |
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| Authors: | JIA Chang-ping HUANG Xue-li LI Yun and FENG Fang |
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| Institution: | Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China |
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| Abstract: | The alkaloids in Zanthoxylum nitidum were identified by HPLC-DAD/ESI-Q-TOF-MS. Separation was performed on a Hanbon C18 column with acetonitrile(with 0.1% formic acid) and water(with 0.1% formic acid) as mobile phase. Based on the high-resolution mass information, MS/MS fragmentation behaviors and chemical components from literatures, 48 components were identified or tentatively characterized including 6 new compounds. This work could be useful for the quality control and further studies of the plant. |
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| Keywords: | Zanthoxylum nitidum HPLC-DAD/ESI-Q-TOF-MS alkaloids |
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